3v3o

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[[Image:3v3o.jpg|left|200px]]
 
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{{STRUCTURE_3v3o| PDB=3v3o | SCENE= }}
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==Crystal structure of TetX2 T280A: an adaptive mutant in complex with tigecycline==
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<StructureSection load='3v3o' size='340' side='right'caption='[[3v3o]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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===Crystal structure of TetX2 T280A: an adaptive mutant in complex with tigecycline===
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3v3o]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron Bacteroides thetaiotaomicron]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V3O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V3O FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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==About this Structure==
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=T1C:TIGECYCLINE'>T1C</scene></td></tr>
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[[3v3o]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron Bacteroides thetaiotaomicron]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V3O OCA].
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v3o OCA], [https://pdbe.org/3v3o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v3o RCSB], [https://www.ebi.ac.uk/pdbsum/3v3o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v3o ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TETX_BACT4 TETX_BACT4] An FAD-requiring monooxygenase active on tetracycline antibiotic derivatives, which leads to their inactivation (PubMed:15452119, PubMed:16128584). Hydroxylates carbon 11a of oxytetracycline and tigecycline (PubMed:15452119, PubMed:26097034). Acts on many tetracycline analogs (chlorotetracycline, demeclocycline, doxycycline, minocycline, oxytetracyclinee), probably by monooxygenization (PubMed:15452119, PubMed:16128584). Tigecycline, a new generation tetracycline antibiotic, is rendered less effective against E.coli by this monooxygenation, is much weaker at inhibiting translation in vitro and binds Mg(2+) considerably less well (PubMed:16128584, PubMed:26097034). Expression in E.coli BW25113 reduces its growth rate about 5%. The reaction probably proceeds by FAD reduction by NADPH and, second, hydroxylation of antibiotic in a ping-pong mechanism (PubMed:23236139). Degrades chlortetracycline, probably by monooxygenation (PubMed:15452119, PubMed:28481346). Slowly oxidizes anhydrotetracycline, the final substrate in tetracycline biosynthesis (PubMed:26097034).[HAMAP-Rule:MF_00845]<ref>PMID:15452119</ref> <ref>PMID:16128584</ref> <ref>PMID:23236139</ref> <ref>PMID:26097034</ref> <ref>PMID:28481346</ref>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bacteroides thetaiotaomicron]]
[[Category: Bacteroides thetaiotaomicron]]
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[[Category: Shamoo, Y.]]
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[[Category: Large Structures]]
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[[Category: Walkiewicz, K.]]
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[[Category: Shamoo Y]]
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[[Category: Oxidoreductase-antibiotic complex]]
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[[Category: Walkiewicz K]]
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[[Category: Rossmann fold]]
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[[Category: Tetracycline degrading monooxygenase]]
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Current revision

Crystal structure of TetX2 T280A: an adaptive mutant in complex with tigecycline

PDB ID 3v3o

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