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2hyp

From Proteopedia

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[[Image:2hyp.png|left|200px]]
 
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{{STRUCTURE_2hyp| PDB=2hyp | SCENE= }}
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==Crystal structure of Rv0805 D66A mutant==
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<StructureSection load='2hyp' size='340' side='right'caption='[[2hyp]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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===Crystal structure of Rv0805 D66A mutant===
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2hyp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HYP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HYP FirstGlance]. <br>
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{{ABSTRACT_PUBMED_17059828}}
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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==About this Structure==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hyp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hyp OCA], [https://pdbe.org/2hyp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hyp RCSB], [https://www.ebi.ac.uk/pdbsum/2hyp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hyp ProSAT]</span></td></tr>
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[[2hyp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HYP OCA].
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</table>
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== Function ==
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==Reference==
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[https://www.uniprot.org/uniprot/CNPD3_MYCTU CNPD3_MYCTU] Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP (PubMed:16313172, PubMed:18757371). Can use 2',3'-cAMP, 2',3'-cGMP, 3',5'-cAMP, 3',5'-cGMP and 3',5'-cUMP (PubMed:16313172, PubMed:18757371, PubMed:19801656). Hydrolysis of 2',3'-cAMP produces a mixture of 3'-AMP (major product) and 2'-AMP (minor product) (PubMed:18757371). In vitro, is 150-fold more active in hydrolyzing 2',3'-cAMP than 3',5'-cAMP (PubMed:18757371). Can also hydrolyze the model substrates p-nitrophenyl phosphate (pNPP), bis-(p-nitrophenyl phosphate) (bis(pNPP)) and p-nitrophenyl phenylphosphonate (pNPPP) (PubMed:16313172, PubMed:18757371, PubMed:19801656). Plays an important regulatory role in modulating the intracellular concentration of cAMP, thereby influencing cAMP-dependent processes (PubMed:16313172). May play a role in pathogenicity, not only by hydrolyzing cAMP, but also by altering properties of the cell wall (PubMed:19801656).<ref>PMID:16313172</ref> <ref>PMID:18757371</ref> <ref>PMID:19801656</ref> Overexpression elicits a transcriptional response that is independent of the phosphodiesterase activity. It does not alter the levels of cAMP-CRP regulated genes, even though cAMP levels are reduced in cells.<ref>PMID:23835087</ref>
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<ref group="xtra">PMID:017059828</ref><ref group="xtra">PMID:016313172</ref><references group="xtra"/>
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== Evolutionary Conservation ==
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[[Category: 3',5'-cyclic-nucleotide phosphodiesterase]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Mycobacterium tuberculosis h37rv]]
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Check<jmol>
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[[Category: Capuder, M.]]
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<jmolCheckbox>
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[[Category: Draskovic, P.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hy/2hyp_consurf.spt"</scriptWhenChecked>
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[[Category: Lamba, D.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Podobnik, M.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Shenoy, A R.]]
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</jmolCheckbox>
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[[Category: Visweswariah, S S.]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hyp ConSurf].
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[[Category: Bi-nuclear active site]]
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<div style="clear:both"></div>
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[[Category: Camp]]
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== References ==
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[[Category: Hydrolase]]
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<references/>
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[[Category: Metallophosphoesterase]]
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__TOC__
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[[Category: Phosphodiesterase]]
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Capuder M]]
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[[Category: Draskovic P]]
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[[Category: Lamba D]]
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[[Category: Podobnik M]]
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[[Category: Shenoy AR]]
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[[Category: Visweswariah SS]]

Current revision

Crystal structure of Rv0805 D66A mutant

PDB ID 2hyp

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