2w6r

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[[Image:2w6r.png|left|200px]]
 
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{{STRUCTURE_2w6r| PDB=2w6r | SCENE= }}
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==Crystal structure of an artificial (ba)8-barrel protein designed from identical half barrels==
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<StructureSection load='2w6r' size='340' side='right'caption='[[2w6r]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2w6r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W6R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W6R FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w6r OCA], [https://pdbe.org/2w6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w6r RCSB], [https://www.ebi.ac.uk/pdbsum/2w6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w6r ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HIS6_THEMA HIS6_THEMA] IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.[HAMAP-Rule:MF_01013]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w6/2w6r_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w6r ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ample evidence suggests that the ubiquitous (betaalpha)(8)-barrel enzyme fold has evolved by the duplication and fusion of an ancestral (betaalpha)(4)-half-barrel. To reconstruct this process in the laboratory with a model protein, we earlier fused two copies of the C-terminal half-barrel HisF-C of imidazole glycerol phosphate synthase (HisF) and stepwise stabilized the resulting HisF-CC construct. We now further increased its stability and solubility by introducing two additional amino acid exchanges, which allowed us to crystallize the resulting artificial (betaalpha)(8)-barrel protein HisF-C***C. The analysis of its X-ray structure at 2.1 A resolution reveals a striking similarity to wild-type HisF, helps us to understand its improved stability, and provides further insights into the evolution of (betaalpha)(8)-barrel proteins.
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===CRYSTAL STRUCTURE OF AN ARTIFICIAL (BA)8-BARREL PROTEIN DESIGNED FROM IDENTICAL HALF BARRELS===
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High-Resolution Crystal Structure of an Artificial (betaalpha)(8)-Barrel Protein Designed from Identical Half-Barrels (dagger) (double dagger).,Hocker B, Lochner A, Seitz T, Claren J, Sterner R Biochemistry. 2009 Jan 23. PMID:19166324<ref>PMID:19166324</ref>
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{{ABSTRACT_PUBMED_19166324}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2w6r" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[2w6r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W6R OCA].
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*[[IGPS 3D structures|IGPS 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019166324</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
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[[Category: Claren, J.]]
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[[Category: Claren J]]
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[[Category: Hocker, B.]]
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[[Category: Hocker B]]
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[[Category: Lochner, A.]]
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[[Category: Lochner A]]
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[[Category: Seitz, T.]]
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[[Category: Seitz T]]
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[[Category: Sterner, R.]]
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[[Category: Sterner R]]
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[[Category: Cobalamin]]
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[[Category: Fusion protein]]
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[[Category: Lyase]]
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[[Category: Novel fold]]
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[[Category: Precorrin]]
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[[Category: Vitamin b12]]
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Current revision

Crystal structure of an artificial (ba)8-barrel protein designed from identical half barrels

PDB ID 2w6r

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