2ve4

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:58, 9 May 2024) (edit) (undo)
 
(6 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2ve4.png|left|200px]]
 
-
{{STRUCTURE_2ve4| PDB=2ve4 | SCENE= }}
+
==Substrate free cyanobacterial CYP120A1==
 +
<StructureSection load='2ve4' size='340' side='right'caption='[[2ve4]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2ve4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VE4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VE4 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ve4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ve4 OCA], [https://pdbe.org/2ve4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ve4 RCSB], [https://www.ebi.ac.uk/pdbsum/2ve4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ve4 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CP120_SYNY3 CP120_SYNY3]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ve/2ve4_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ve4 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The crystal structures of substrate-free and all- trans-retinoic acid-bound CYP120A1 from Synechocystis sp. PCC 6803 were determined at 2.4 and 2.1 A resolution, respectively, representing the first structural characterization of a cyanobacterial P450. Features of CYP120A1 not observed in other P450 structures include an aromatic ladder flanking the channel leading to the active site and a triple-glycine motif within SRS5. Using spectroscopic methods, CYP120A1 is shown to bind 13- cis-retinoic acid, 9- cis-retinoic acid, and retinal with high affinity and dissociation constants of less than 1 microM. Metabolism of retinoic acid by CYP120A1 suggests that CYP120A1 hydroxylates a variety of retinoid derivatives in vivo. On the basis of the retinoic acid-bound CYP120A1 crystal structure, we propose that either carbon 2 or the methyl groups (C16 or C17) of the beta-ionone ring are modified by CYP120A1.
-
===SUBSTRATE FREE CYANOBACTERIAL CYP120A1===
+
Crystal Structures of Substrate-Free and Retinoic Acid-Bound Cyanobacterial Cytochrome P450 CYP120A1.,Kuhnel K, Ke N, Cryle MJ, Sligar SG, Schuler MA, Schlichting I Biochemistry. 2008 May 31. PMID:18512957<ref>PMID:18512957</ref>
-
{{ABSTRACT_PUBMED_18512957}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 2ve4" style="background-color:#fffaf0;"></div>
-
[[2ve4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VE4 OCA].
+
== References ==
-
 
+
<references/>
-
==Reference==
+
__TOC__
-
<ref group="xtra">PMID:018512957</ref><references group="xtra"/>
+
</StructureSection>
-
[[Category: Synechocystis sp.]]
+
[[Category: Large Structures]]
-
[[Category: Ke, N.]]
+
[[Category: Synechocystis sp. PCC 6803]]
-
[[Category: Kuhnel, K.]]
+
[[Category: Ke N]]
-
[[Category: Schlichting, I.]]
+
[[Category: Kuhnel K]]
-
[[Category: Schuler, M A.]]
+
[[Category: Schlichting I]]
-
[[Category: Sligar, S G.]]
+
[[Category: Schuler MA]]
-
[[Category: Heme]]
+
[[Category: Sligar SG]]
-
[[Category: Iron]]
+
-
[[Category: Metal-binding]]
+
-
[[Category: Monooxygenase]]
+
-
[[Category: Oxidoreductase]]
+

Current revision

Substrate free cyanobacterial CYP120A1

PDB ID 2ve4

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ve4"
Personal tools