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1zia

From Proteopedia

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[[Image:1zia.gif|left|200px]]<br /><applet load="1zia" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1zia, resolution 1.54&Aring;" />
 
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'''OXIDIZED PSEUDOAZURIN'''<br />
 
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==Overview==
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==OXIDIZED PSEUDOAZURIN==
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New crystals of a blue copper protein, pseudoazurin from denitrifier Achromobacter cycloclastes IAM1013, have been obtained by means of vapor diffusion with ammonium sulfate as a precipitant at pH 6.0 and 4 degrees C. The crystals belong to the orthorhombic system, space group P2(1)2(1)2(1), with unit cell dimensions of a = 56.69(2), b = 61.53(2), and c = 30.20(1) A. The asymmetric unit includes one molecule of pseudoazurin with a Vm value of 2.04 A3/Da. The crystals are so stable against X-ray irradiation that a complete data set up to 1.54 A has been collected using a single native crystal. Solution of the structure was performed by means of the Patterson search techniques, and the current crystallographic R-factor is 17.5% at 3.0 A resolution. Refinement at higher resolution is in progress.
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<StructureSection load='1zia' size='340' side='right'caption='[[1zia]], [[Resolution|resolution]] 1.54&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1zia]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Achromobacter_cycloclastes Achromobacter cycloclastes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZIA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZIA FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.54&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zia OCA], [https://pdbe.org/1zia PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zia RCSB], [https://www.ebi.ac.uk/pdbsum/1zia PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zia ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AZUP_ACHCY AZUP_ACHCY] This soluble electron transfer copper protein is required for the inactivation of copper-containing nitrite reductase in the presence of oxygen.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zi/1zia_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zia ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1ZIA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Achromobacter_cycloclastes Achromobacter cycloclastes] with <scene name='pdbligand=CU:'>CU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=COP:Cu+Coordination'>COP</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZIA OCA].
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*[[Pseudoazurin|Pseudoazurin]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystallization and preliminary X-ray studies on pseudoazurin from Achromobacter cycloclastes IAM1013., Inoue T, Nishio N, Kai Y, Harada S, Ohshiro Y, Suzuki S, Kohzuma T, Shidara S, Iwasaki H, J Biochem. 1993 Dec;114(6):761-2. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8138527 8138527]
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[[Category: Achromobacter cycloclastes]]
[[Category: Achromobacter cycloclastes]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Hamanaka, S.]]
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[[Category: Hamanaka S]]
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[[Category: Harada, S.]]
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[[Category: Harada S]]
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[[Category: Inoue, T.]]
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[[Category: Inoue T]]
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[[Category: Iwasaki, H.]]
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[[Category: Iwasaki H]]
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[[Category: Kai, Y.]]
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[[Category: Kai Y]]
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[[Category: Kohzuma, T.]]
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[[Category: Kohzuma T]]
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[[Category: Nishio, N.]]
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[[Category: Nishio N]]
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[[Category: Shidara, S.]]
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[[Category: Shidara S]]
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[[Category: Shimomura, T.]]
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[[Category: Shimomura T]]
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[[Category: Suzuki, S.]]
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[[Category: Suzuki S]]
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[[Category: CU]]
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[[Category: cuproprotein]]
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[[Category: electron transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:15:49 2008''
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Current revision

OXIDIZED PSEUDOAZURIN

PDB ID 1zia

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