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1hjs

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[[Image:1hjs.gif|left|200px]]<br />
 
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<applet load="1hjs" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1hjs, resolution 1.87&Aring;" />
 
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'''STRUCTURE OF TWO FUNGAL BETA-1,4-GALACTANASES: SEARCHING FOR THE BASIS FOR TEMPERATURE AND PH OPTIMUM.'''<br />
 
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==Overview==
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==Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum.==
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beta-1,4-Galactanases hydrolyze the galactan side chains that are part of, the complex carbohydrate structure of the pectin. They are assigned to, family 53 of the glycoside hydrolases and display significant variations, in their pH and temperature optimum and stability. Two fungal, beta-1,4-galactanases from Myceliophthora thermophila and Humicola, insolens have been cloned and heterologously expressed, and the crystal, structures of the gene products were determined. The structures are, compared to the previously only known family 53 structure of the, galactanase from Aspergillus aculeatus (AAGAL) showing approximately 56%, identity. The M. thermophila and H. insolens galactanases are thermophilic, enzymes and are most active at neutral to basic pH, whereas AAGAL is, mesophilic and most ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12761390 (full description)]]
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<StructureSection load='1hjs' size='340' side='right'caption='[[1hjs]], [[Resolution|resolution]] 1.87&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1hjs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermothelomyces_thermophilus Thermothelomyces thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HJS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.87&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hjs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjs OCA], [https://pdbe.org/1hjs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hjs RCSB], [https://www.ebi.ac.uk/pdbsum/1hjs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hjs ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GANA_THETO GANA_THETO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/1hjs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hjs ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1HJS is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Corynascus_heterothallicus Corynascus heterothallicus]] with NAG, SO4, EPE and PEG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Arabinogalactan_endo-1,4-beta-galactosidase Arabinogalactan endo-1,4-beta-galactosidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.89 3.2.1.89]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HJS OCA]].
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*[[Beta-1%2C4-galactanase|Beta-1%2C4-galactanase]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum., Le Nours J, Ryttersgaard C, Lo Leggio L, Ostergaard PR, Borchert TV, Christensen LL, Larsen S, Protein Sci. 2003 Jun;12(6):1195-204. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12761390 12761390]
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[[Category: Large Structures]]
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[[Category: Arabinogalactan endo-1,4-beta-galactosidase]]
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[[Category: Thermothelomyces thermophilus]]
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[[Category: Corynascus heterothallicus]]
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[[Category: Borchert TV]]
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[[Category: Single protein]]
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[[Category: Christensen LLH]]
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[[Category: Borchert, T.V.]]
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[[Category: Larsen S]]
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[[Category: Christensen, L.L.H.]]
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[[Category: Le Nours J]]
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[[Category: Larsen, S.]]
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[[Category: Lo Leggio L]]
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[[Category: Leggio, L.Lo.]]
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[[Category: Ostergaard PR]]
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[[Category: Nours, J.Le.]]
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[[Category: Ryttersgaard C]]
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[[Category: Ostergaard, P.R.]]
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[[Category: Ryttersgaard, C.]]
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[[Category: EPE]]
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[[Category: NAG]]
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[[Category: PEG]]
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[[Category: SO4]]
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[[Category: 4-galactanases]]
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[[Category: alkalophile]]
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[[Category: beta-1]]
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[[Category: clan gh-a]]
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[[Category: family 53 glycoside hydrolase]]
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[[Category: hydrolase]]
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[[Category: ph optimum]]
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[[Category: thermophile]]
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[[Category: thermostability]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:37:07 2007''
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Current revision

Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum.

PDB ID 1hjs

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