1ztm
From Proteopedia
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| - | [[Image:1ztm.gif|left|200px]]<br /><applet load="1ztm" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1ztm, resolution 3.05Å" /> | ||
| - | '''Structure of the Uncleaved Paramyxovirus (hPIV3) Fusion Protein'''<br /> | ||
| - | == | + | ==Structure of the Uncleaved Paramyxovirus (hPIV3) Fusion Protein== |
| + | <StructureSection load='1ztm' size='340' side='right'caption='[[1ztm]], [[Resolution|resolution]] 3.05Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZTM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZTM FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.05Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ztm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ztm OCA], [https://pdbe.org/1ztm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ztm RCSB], [https://www.ebi.ac.uk/pdbsum/1ztm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ztm ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zt/1ztm_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ztm ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
Class I viral fusion proteins share common mechanistic and structural features but little sequence similarity. Structural insights into the protein conformational changes associated with membrane fusion are based largely on studies of the influenza virus hemagglutinin in pre- and postfusion conformations. Here, we present the crystal structure of the secreted, uncleaved ectodomain of the paramyxovirus, human parainfluenza virus 3 fusion (F) protein, a member of the class I viral fusion protein group. The secreted human parainfluenza virus 3 F forms a trimer with distinct head, neck, and stalk regions. Unexpectedly, the structure reveals a six-helix bundle associated with the postfusion form of F, suggesting that the anchor-minus ectodomain adopts a conformation largely similar to the postfusion state. The transmembrane anchor domains of F may therefore profoundly influence the folding energetics that establish and maintain a metastable, prefusion state. | Class I viral fusion proteins share common mechanistic and structural features but little sequence similarity. Structural insights into the protein conformational changes associated with membrane fusion are based largely on studies of the influenza virus hemagglutinin in pre- and postfusion conformations. Here, we present the crystal structure of the secreted, uncleaved ectodomain of the paramyxovirus, human parainfluenza virus 3 fusion (F) protein, a member of the class I viral fusion protein group. The secreted human parainfluenza virus 3 F forms a trimer with distinct head, neck, and stalk regions. Unexpectedly, the structure reveals a six-helix bundle associated with the postfusion form of F, suggesting that the anchor-minus ectodomain adopts a conformation largely similar to the postfusion state. The transmembrane anchor domains of F may therefore profoundly influence the folding energetics that establish and maintain a metastable, prefusion state. | ||
| - | + | Structure of the uncleaved ectodomain of the paramyxovirus (hPIV3) fusion protein.,Yin HS, Paterson RG, Wen X, Lamb RA, Jardetzky TS Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9288-93. Epub 2005 Jun 17. PMID:15964978<ref>PMID:15964978</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 1ztm" style="background-color:#fffaf0;"></div> |
| - | + | == References == | |
| - | [[Category: Jardetzky | + | <references/> |
| - | [[Category: Lamb | + | __TOC__ |
| - | [[Category: Paterson | + | </StructureSection> |
| - | [[Category: Wen | + | [[Category: Large Structures]] |
| - | [[Category: Yin | + | [[Category: Jardetzky TS]] |
| - | + | [[Category: Lamb RA]] | |
| - | + | [[Category: Paterson RG]] | |
| - | + | [[Category: Wen X]] | |
| - | + | [[Category: Yin HS]] | |
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| - | + | ||
| - | + | ||
Current revision
Structure of the Uncleaved Paramyxovirus (hPIV3) Fusion Protein
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Categories: Large Structures | Jardetzky TS | Lamb RA | Paterson RG | Wen X | Yin HS
