3f6n
From Proteopedia
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| - | [[Image:3f6n.png|left|200px]] | ||
| - | + | ==Crystal structure of the virion-associated protein P3 from Caulimovirus== | |
| + | <StructureSection load='3f6n' size='340' side='right'caption='[[3f6n]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3f6n]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Cauliflower_mosaic_virus_(STRAIN_STRASBOURG) Cauliflower mosaic virus (STRAIN STRASBOURG)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F6N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3F6N FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3f6n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f6n OCA], [https://pdbe.org/3f6n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3f6n RCSB], [https://www.ebi.ac.uk/pdbsum/3f6n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3f6n ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/VAP_CAMVS VAP_CAMVS] Plays a role in virus cell-to-cell and plant-to-plant transmission. Interacts with virion icosahedral capsid and movement protein, thereby facilitating virion cell-to-cell transmission throug plasmodesmata opned by viral movement protein. Also interacts with aphid transmission factor, attaching the virion to aphid stylet when the animal feeds on an virus infected plant. Aphid saliva may later detach the virion, inducing release of infectious particles when the animal feeds on a new plant.<ref>PMID:11324752</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f6/3f6n_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3f6n ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cauliflower mosaic virus (CaMV) is transmitted from plant to plant through a seemingly simple interaction with insect vectors. This process involves an aphid receptor and two viral proteins, P2 and P3. P2 binds to both the aphid receptor and P3, itself tightly associated with the virus particle, with the ensemble forming a transmissible viral complex. Here, we describe the conformations of both unliganded CaMV P3 protein and its virion-associated form. X-ray crystallography revealed that the N-terminal domain of unliganded P3 is a tetrameric parallel coiled coil with a unique organization showing two successive four-stranded subdomains with opposite supercoiling handedness stabilized by a ring of interchain disulfide bridges. A structural model of virus-liganded P3 proteins, folding as an antiparallel coiled-coil network coating the virus surface, was derived from molecular modeling. Our results highlight the structural and biological versatility of this coiled-coil structure and provide new insights into the molecular mechanisms involved in CaMV acquisition and transmission by the insect vector. | ||
| - | + | Structural insights into the molecular mechanisms of cauliflower mosaic virus transmission by its insect vector.,Hoh F, Uzest M, Drucker M, Plisson-Chastang C, Bron P, Blanc S, Dumas C J Virol. 2010 May;84(9):4706-13. Epub 2010 Feb 24. PMID:20181714<ref>PMID:20181714</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 3f6n" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | < | + | </StructureSection> |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Blanc | + | [[Category: Blanc S]] |
| - | [[Category: Dumas | + | [[Category: Dumas C]] |
| - | [[Category: Hoh | + | [[Category: Hoh F]] |
| - | [[Category: Uzest | + | [[Category: Uzest M]] |
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Current revision
Crystal structure of the virion-associated protein P3 from Caulimovirus
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Categories: Large Structures | Blanc S | Dumas C | Hoh F | Uzest M
