3f8n

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[[Image:3f8n.png|left|200px]]
 
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{{STRUCTURE_3f8n| PDB=3f8n | SCENE= }}
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==Crystal structure of PerR-Zn-Mn==
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<StructureSection load='3f8n' size='340' side='right'caption='[[3f8n]], [[Resolution|resolution]] 3.15&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3f8n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F8N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3F8N FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3f8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f8n OCA], [https://pdbe.org/3f8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3f8n RCSB], [https://www.ebi.ac.uk/pdbsum/3f8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3f8n ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PERR_BACSU PERR_BACSU] Hydrogen and organic peroxide sensor. Represses the expression of a regulon of peroxide-inducible genes such as katA, ahpC, ahpF, the heme biosynthesis operon (hemAXCDBL), fur, perR, zosA and mrgA.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f8/3f8n_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3f8n ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In Bacillus subtilis, the transcription factor PerR is an iron dependant sensor of H(2)O(2). The sensing mechanism relies on a selective metal catalysed oxidation of two histidine residues of the regulatory site. Here we present the first crystal structure of the active PerR protein in complex with a Mn(2+) ion. In addition, X-ray absorption spectroscopy experiments were performed to characterize the corresponding iron form of the protein. Both studies reveal a penta-coordinate arrangement of the regulatory site that involves three histidines and two aspartates. One of the histidine ligand belongs to the N-terminal domain. Binding of this residue to the regulatory metal allows the protein to adopt a caliper-like conformation suited to DNA binding. Since this histidine is conserved in all PerR and a vast majority of Fur proteins, it is likely that the allosteric switch induced by the regulatory metal is general for this family of metalloregulators.
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===Crystal structure of PerR-Zn-Mn===
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Structural characterization of the active form of PerR: insights into the metal-induced activation of PerR and Fur proteins for DNA binding.,Jacquamet L, Traore DA, Ferrer JL, Proux O, Testemale D, Hazemann JL, Nazarenko E, El Ghazouani A, Caux-Thang C, Duarte V, Latour JM Mol Microbiol. 2009 Jul;73(1):20-31. Epub 2009 Jun 8. PMID:19508285<ref>PMID:19508285</ref>
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{{ABSTRACT_PUBMED_19508285}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3f8n" style="background-color:#fffaf0;"></div>
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[[3f8n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F8N OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:019508285</ref><references group="xtra"/>
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Duarte, V.]]
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[[Category: Large Structures]]
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[[Category: Ferrer, J L.]]
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[[Category: Duarte V]]
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[[Category: Jacquamet, L.]]
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[[Category: Ferrer J-L]]
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[[Category: Latour, J M.]]
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[[Category: Jacquamet L]]
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[[Category: Traore, D A.K.]]
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[[Category: Latour J-M]]
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[[Category: Dna binding protein]]
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[[Category: Traore DAK]]
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[[Category: Dna-binding]]
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[[Category: Helix-turn-helix]]
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[[Category: Manganese]]
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[[Category: Oxidation]]
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[[Category: Repressor]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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Current revision

Crystal structure of PerR-Zn-Mn

PDB ID 3f8n

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