3f70

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[[Image:3f70.png|left|200px]]
 
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{{STRUCTURE_3f70| PDB=3f70 | SCENE= }}
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==Crystal structure of L3MBTL2-H4K20me1 complex==
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<StructureSection load='3f70' size='340' side='right'caption='[[3f70]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3f70]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3dbb 3dbb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F70 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3F70 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLZ:N-METHYL-LYSINE'>MLZ</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3f70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f70 OCA], [https://pdbe.org/3f70 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3f70 RCSB], [https://www.ebi.ac.uk/pdbsum/3f70 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3f70 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LMBL2_HUMAN LMBL2_HUMAN] Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Its association with a chromatin-remodeling complex suggests that it may contribute to prevent expression of genes that trigger the cell into mitosis. Binds to monomethylated and dimethylated 'Lys-20' on histone H4. Binds histone H3 peptides that are monomethylated or dimethylated on 'Lys-4', 'Lys-9' or 'Lys-27'.<ref>PMID:19233876</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f7/3f70_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3f70 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The MBT repeat has been recently identified as a key domain capable of methyl-lysine histone recognition. Functional work has pointed to a role for MBT domain-containing proteins in transcriptional repression of developmental control genes such as Hox genes. In this study, L3MBTL2, a human homolog of Drosophila Sfmbt critical for Hox gene silencing, is demonstrated to preferentially recognize lower methylation states of several histone-derived peptides through its fourth MBT repeat. High-resolution crystallographic analysis of the four MBT repeats of this protein reveals its unique asymmetric rhomboid architecture, as well as binding mechanism, which preclude the interaction of the first three MBT repeats with methylated peptides. Structural elucidation of an L3MBTL2-H4K20me1 complex and comparison with other MBT-histone peptide complexes also suggests that an absence of distinct surface contours surrounding the methyl-lysine-binding pocket may underlie the lack of sequence specificity observed for members of this protein family.
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===Crystal structure of L3MBTL2-H4K20me1 complex===
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Methylation-state-specific recognition of histones by the MBT repeat protein L3MBTL2.,Guo Y, Nady N, Qi C, Allali-Hassani A, Zhu H, Pan P, Adams-Cioaba MA, Amaya MF, Dong A, Vedadi M, Schapira M, Read RJ, Arrowsmith CH, Min J Nucleic Acids Res. 2009 Apr;37(7):2204-10. Epub 2009 Feb 20. PMID:19233876<ref>PMID:19233876</ref>
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{{ABSTRACT_PUBMED_19233876}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3f70" style="background-color:#fffaf0;"></div>
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[[3f70]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3dbb 3dbb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F70 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:019233876</ref><references group="xtra"/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Allali-Hassani, A.]]
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[[Category: Large Structures]]
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[[Category: Arrowsmith, C H.]]
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[[Category: Allali-Hassani A]]
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[[Category: Botchkarev, A.]]
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[[Category: Arrowsmith CH]]
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[[Category: Bountra, C.]]
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[[Category: Botchkarev A]]
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[[Category: Crombet, L.]]
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[[Category: Bountra C]]
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[[Category: Dong, A.]]
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[[Category: Crombet L]]
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[[Category: Edwards, A M.]]
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[[Category: Dong A]]
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[[Category: Guo, Y.]]
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[[Category: Edwards AM]]
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[[Category: Kozieradzki, I.]]
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[[Category: Guo Y]]
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[[Category: Loppnau, P.]]
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[[Category: Kozieradzki I]]
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[[Category: Mackenzie, F.]]
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[[Category: Loppnau P]]
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[[Category: Min, J.]]
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[[Category: Mackenzie F]]
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[[Category: Pan, P.]]
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[[Category: Min J]]
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[[Category: Qi, C.]]
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[[Category: Pan P]]
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[[Category: Read, R.]]
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[[Category: Qi C]]
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[[Category: SGC, Structural Genomics Consortium.]]
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[[Category: Read R]]
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[[Category: Vedadi, M.]]
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[[Category: Vedadi M]]
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[[Category: Weigelt, J.]]
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[[Category: Weigelt J]]
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[[Category: Zhu, H.]]
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[[Category: Zhu H]]
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[[Category: Chromatin regulator]]
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[[Category: Mbt]]
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[[Category: Metal-binding]]
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[[Category: Nucleus]]
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[[Category: Phosphoprotein]]
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[[Category: Sgc]]
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[[Category: Structural genomic]]
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[[Category: Structural genomics consortium]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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[[Category: Transcription regulator]]
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[[Category: Zinc-finger]]
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Current revision

Crystal structure of L3MBTL2-H4K20me1 complex

PDB ID 3f70

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