3fhy

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[[Image:3fhy.png|left|200px]]
 
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{{STRUCTURE_3fhy| PDB=3fhy | SCENE= }}
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==Crystal structure of D235N mutant of human pyridoxal kinase==
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<StructureSection load='3fhy' size='340' side='right'caption='[[3fhy]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3fhy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FHY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FHY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fhy OCA], [https://pdbe.org/3fhy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fhy RCSB], [https://www.ebi.ac.uk/pdbsum/3fhy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fhy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PDXK_HUMAN PDXK_HUMAN] Required for synthesis of pyridoxal-5-phosphate from vitamin B6.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Pyridoxal kinase catalyzes the phosphorylation of pyridoxal (PL) to pyridoxal 5'-phosphate (PLP). A D235A variant shows 7-fold and 15-fold decreases in substrate affinity and activity, respectively. A D235N variant shows approximately 2-fold decrease in both PL affinity and activity. The crystal structure of D235A (2.5 A) shows bound ATP, PL and PLP, while D235N (2.3 A) shows bound ATP and sulfate. These results document the role of Asp235 in PL kinase activity. The observation that the active site of PL kinase can accommodate both ATP and PLP suggests that formation of a ternary Enz.PLP.ATP complex could occur in the wild-type enzyme, consistent with severe MgATP substrate inhibition of PL kinase in the presence of PLP.
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===Crystal structure of D235N mutant of human pyridoxal kinase===
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Kinetic and structural studies of the role of the active site residue Asp235 of human pyridoxal kinase.,Gandhi AK, Ghatge MS, Musayev FN, Sease A, Aboagye SO, di Salvo ML, Schirch V, Safo MK Biochem Biophys Res Commun. 2009 Mar 27;381(1):12-5. Epub 2009 Feb 4. PMID:19351586<ref>PMID:19351586</ref>
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{{ABSTRACT_PUBMED_19351586}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3fhy" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[3fhy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FHY OCA].
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*[[Pyridoxal kinase|Pyridoxal kinase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019351586</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Pyridoxal kinase]]
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[[Category: Large Structures]]
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[[Category: Gandhi, A K.]]
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[[Category: Di Salvo ML]]
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[[Category: Ghatge, M.]]
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[[Category: Gandhi AK]]
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[[Category: Musayev, F N.]]
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[[Category: Ghatge M]]
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[[Category: Safo, M K.]]
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[[Category: Musayev FN]]
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[[Category: Salvo, M L.Di.]]
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[[Category: Safo MK]]
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[[Category: Schirch, V.]]
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[[Category: Schirch V]]
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[[Category: Atp complex]]
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[[Category: Atp-binding]]
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[[Category: Beta sheet with alpha helix]]
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[[Category: Kinase]]
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[[Category: Metal ion]]
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[[Category: Metal-binding]]
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[[Category: Nucleotide-binding]]
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[[Category: Transferase]]
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Current revision

Crystal structure of D235N mutant of human pyridoxal kinase

PDB ID 3fhy

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