3gbd
From Proteopedia
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| - | [[Image:3gbd.png|left|200px]] | ||
| - | + | ==Crystal structure of the isomaltulose synthase SmuA from Protaminobacter rubrum== | |
| + | <StructureSection load='3gbd' size='340' side='right'caption='[[3gbd]], [[Resolution|resolution]] 1.95Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3gbd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Serratia_plymuthica Serratia plymuthica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GBD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GBD FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gbd OCA], [https://pdbe.org/3gbd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gbd RCSB], [https://www.ebi.ac.uk/pdbsum/3gbd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gbd ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/D0VX20_SERPL D0VX20_SERPL] | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gb/3gbd_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gbd ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The healthy sweetener isomaltulose is industrially produced from the conversion of sucrose by the sucrose isomerase SmuA from Protaminobacter rubrum. Crystal structures of SmuA in native and deoxynojirimycin complexed forms completed with modeling studies unravel the characteristics of the isomaltulose synthases catalytic pocket and their substrate binding mode. Comparison with the trehalulose synthase MutB highlights the role of Arg(298) and Arg(306) active site residues and surface charges in controlling product specificity of sucrose isomerases (isomaltulose versus trehalulose). The results provide a rationale for the specific design of optimized enzymes. | ||
| - | + | Structural determinants of product specificity of sucrose isomerases.,Ravaud S, Robert X, Watzlawick H, Haser R, Mattes R, Aghajari N FEBS Lett. 2009 Jun 18;583(12):1964-8. Epub 2009 May 8. PMID:19427862<ref>PMID:19427862</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 3gbd" style="background-color:#fffaf0;"></div> | ||
| - | == | + | ==See Also== |
| - | [[ | + | *[[Trehalulose synthase|Trehalulose synthase]] |
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Aghajari | + | [[Category: Serratia plymuthica]] |
| - | [[Category: Haser | + | [[Category: Aghajari N]] |
| - | [[Category: Ravaud | + | [[Category: Haser R]] |
| - | [[Category: Robert | + | [[Category: Ravaud S]] |
| - | + | [[Category: Robert X]] | |
| - | + | ||
| - | + | ||
| - | + | ||
Current revision
Crystal structure of the isomaltulose synthase SmuA from Protaminobacter rubrum
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