2j80

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[[Image:2j80.png|left|200px]]
 
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{{STRUCTURE_2j80| PDB=2j80 | SCENE= }}
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==Structure of Citrate-bound Periplasmic Domain of Sensor Histidine Kinase CitA==
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<StructureSection load='2j80' size='340' side='right'caption='[[2j80]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2j80]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J80 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J80 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j80 OCA], [https://pdbe.org/2j80 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j80 RCSB], [https://www.ebi.ac.uk/pdbsum/2j80 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j80 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CITA_KLEPN CITA_KLEPN] Member of the two-component regulatory system CitA/CitB. Probably activates CitB by phosphorylation. The periplasmic domain binds H-citrate(2-), which is essential for induction of the citrate-fermentation genes.<ref>PMID:10447894</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j8/2j80_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j80 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Sensor histidine kinases of two-component signal-transduction systems are essential for bacteria to adapt to variable environmental conditions. However, despite their prevalence, it is not well understood how extracellular signals such as ligand binding regulate the activity of these sensor kinases. CitA is the sensor histidine kinase in Klebsiella pneumoniae that regulates the transport and anaerobic metabolism of citrate in response to its extracellular concentration. We report here the X-ray structures of the periplasmic sensor domain of CitA in the citrate-free and citrate-bound states. A comparison of the two structures shows that ligand binding causes a considerable contraction of the sensor domain. This contraction may represent the molecular switch that activates transmembrane signaling in the receptor.
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===STRUCTURE OF CITRATE-BOUND PERIPLASMIC DOMAIN OF SENSOR HISTIDINE KINASE CITA===
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A ligand-induced switch in the periplasmic domain of sensor histidine kinase CitA.,Sevvana M, Vijayan V, Zweckstetter M, Reinelt S, Madden DR, Herbst-Irmer R, Sheldrick GM, Bott M, Griesinger C, Becker S J Mol Biol. 2008 Mar 21;377(2):512-23. Epub 2008 Jan 16. PMID:18258261<ref>PMID:18258261</ref>
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{{ABSTRACT_PUBMED_18258261}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2j80" style="background-color:#fffaf0;"></div>
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[[2j80]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J80 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:018258261</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Histidine kinase]]
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[[Category: Klebsiella pneumoniae]]
[[Category: Klebsiella pneumoniae]]
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[[Category: Becker, S.]]
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[[Category: Large Structures]]
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[[Category: Bott, M.]]
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[[Category: Becker S]]
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[[Category: Griesinger, C.]]
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[[Category: Bott M]]
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[[Category: Madden, D R.]]
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[[Category: Griesinger C]]
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[[Category: Reinelt, S.]]
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[[Category: Madden DR]]
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[[Category: Sevvana, M.]]
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[[Category: Reinelt S]]
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[[Category: Sheldrick, G M.]]
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[[Category: Sevvana M]]
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[[Category: Vijayan, V.]]
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[[Category: Sheldrick GM]]
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[[Category: Zweckstetter, M.]]
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[[Category: Vijayan V]]
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[[Category: Phosphorylation]]
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[[Category: Zweckstetter M]]
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[[Category: Signal transduction]]
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[[Category: Transferase]]
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[[Category: Transmembrane]]
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[[Category: Two-component regulatory system]]
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Current revision

Structure of Citrate-bound Periplasmic Domain of Sensor Histidine Kinase CitA

PDB ID 2j80

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