3fxb

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[[Image:3fxb.png|left|200px]]
 
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{{STRUCTURE_3fxb| PDB=3fxb | SCENE= }}
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==Crystal structure of the ectoine-binding protein UehA==
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<StructureSection load='3fxb' size='340' side='right'caption='[[3fxb]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3fxb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ruegeria_pomeroyi_DSS-3 Ruegeria pomeroyi DSS-3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FXB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4CS:(4S)-2-METHYL-1,4,5,6-TETRAHYDROPYRIMIDINE-4-CARBOXYLIC+ACID'>4CS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fxb OCA], [https://pdbe.org/3fxb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fxb RCSB], [https://www.ebi.ac.uk/pdbsum/3fxb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fxb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UEHA_RUEPO UEHA_RUEPO] Part of the tripartite ATP-independent periplasmic (TRAP) transport system UehABC, which imports both ectoine and 5-hydroxyectoine as nutrients, and not as osmoprotectants. UehA binds both ectoine and 5-hydroxyectoine with high specificity and affinity.<ref>PMID:19362561</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fx/3fxb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fxb ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Substrate-binding proteins or extracellular solute receptors (ESRs) are components of both ABC (ATP binding cassette) and TRAP-T (tripartite ATP-independent periplasmic transporter). The TRAP-T system UehABC from Silicibacter pomeroyi DSS-3 imports the compatible solutes ectoine and 5-hydroxyectoine as nutrients. UehA, the ESR of the UehABC operon, binds both ectoine and 5-hydroxyectoine with high affinity (K(d) values of 1.4+/-0.1 and 1.1+/-0.1 microM, respectively) and delivers them to the TRAP-T complex. The crystal structure of UehA in complex with ectoine was determined at 2.9-A resolution and revealed an overall fold common for all ESR proteins from TRAP systems determined so far. A comparison of the recently described structure of TeaA from Halomonas elongata and an ectoine-binding protein (EhuB) from an ABC transporter revealed a conserved ligand binding mode that involves both directed and cation-pi interactions. Furthermore, a comparison with other known TRAP-T ESRs revealed a helix that might act as a selectivity filter imposing restraints on the ESRs that fine-tune ligand recognition and binding and finally might determine the selection of the cognate substrate.
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===Crystal structure of the ectoine-binding protein UehA===
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The crystal structure of UehA in complex with ectoine-A comparison with other TRAP-T binding proteins.,Lecher J, Pittelkow M, Zobel S, Bursy J, Bonig T, Smits SH, Schmitt L, Bremer E J Mol Biol. 2009 May 29;389(1):58-73. Epub 2009 Apr 10. PMID:19362561<ref>PMID:19362561</ref>
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{{ABSTRACT_PUBMED_19362561}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3fxb" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[3fxb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ruegeria_pomeroyi_dss-3 Ruegeria pomeroyi dss-3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FXB OCA].
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*[[TRAP dicarboxylate transporter%2C DctP subunit|TRAP dicarboxylate transporter%2C DctP subunit]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019362561</ref><references group="xtra"/>
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__TOC__
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[[Category: Ruegeria pomeroyi dss-3]]
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</StructureSection>
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[[Category: Bremer, E.]]
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[[Category: Large Structures]]
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[[Category: Bursy, J.]]
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[[Category: Ruegeria pomeroyi DSS-3]]
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[[Category: Lecher, J.]]
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[[Category: Bremer E]]
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[[Category: Pittelkow, M.]]
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[[Category: Bursy J]]
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[[Category: Schmitt, L.]]
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[[Category: Lecher J]]
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[[Category: Smits, S H.J.]]
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[[Category: Pittelkow M]]
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[[Category: Membrane]]
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[[Category: Schmitt L]]
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[[Category: Periplasmic substrate binding protein]]
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[[Category: Smits SHJ]]
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[[Category: Selectivity helix]]
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[[Category: Transport]]
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[[Category: Transport protein]]
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Current revision

Crystal structure of the ectoine-binding protein UehA

PDB ID 3fxb

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