3fyr

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[[Image:3fyr.png|left|200px]]
 
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{{STRUCTURE_3fyr| PDB=3fyr | SCENE= }}
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==Crystal structure of the sporulation histidine kinase inhibitor Sda from Bacillus subtilis==
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<StructureSection load='3fyr' size='340' side='right'caption='[[3fyr]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3fyr]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FYR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FYR FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.97&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fyr OCA], [https://pdbe.org/3fyr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fyr RCSB], [https://www.ebi.ac.uk/pdbsum/3fyr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fyr ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fy/3fyr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fyr ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the DNA-damage checkpoint inhibitor of sporulation, Sda, from Bacillus subtilis, has been solved by the MAD technique using selenomethionine-substituted protein. The structure closely resembles that previously solved by NMR, as well as the structure of a homologue from Geobacillus stearothermophilus solved in complex with the histidine kinase KinB. The structure contains three molecules in the asymmetric unit. The unusual trimeric arrangement, which lacks simple internal symmetry, appears to be preserved in solution based on an essentially ideal fit to previously acquired scattering data for Sda in solution. This interpretation contradicts previous findings that Sda was monomeric or dimeric in solution. This study demonstrates the difficulties that can be associated with the characterization of small proteins and the value of combining multiple biophysical techniques. It also emphasizes the importance of understanding the physical principles behind these techniques and therefore their limitations.
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===Crystal structure of the sporulation histidine kinase inhibitor Sda from Bacillus subtilis===
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Structure of the sporulation histidine kinase inhibitor Sda from Bacillus subtilis and insights into its solution state.,Jacques DA, Streamer M, Rowland SL, King GF, Guss JM, Trewhella J, Langley DB Acta Crystallogr D Biol Crystallogr. 2009 Jun;65(Pt 6):574-81. Epub 2009, May 15. PMID:19465772<ref>PMID:19465772</ref>
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{{ABSTRACT_PUBMED_19465772}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3fyr" style="background-color:#fffaf0;"></div>
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[[3fyr]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FYR OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:019465772</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Bacillus subtilis]]
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[[Category: Bacillus subtilis subsp. subtilis str. 168]]
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[[Category: Guss, J M.]]
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[[Category: Large Structures]]
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[[Category: Jacques, D A.]]
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[[Category: Guss JM]]
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[[Category: King, G F.]]
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[[Category: Jacques DA]]
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[[Category: Langley, D B.]]
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[[Category: King GF]]
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[[Category: Streamer, M.]]
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[[Category: Langley DB]]
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[[Category: Trewhella, J.]]
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[[Category: Streamer M]]
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[[Category: Alternative initiation]]
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[[Category: Trewhella J]]
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[[Category: Helical hairpin]]
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[[Category: Histidine kinase inhibitor]]
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[[Category: Protein kinase inhibitor]]
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[[Category: Sporulation]]
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[[Category: Sporulation regulation]]
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[[Category: Transferase inhibitor]]
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Current revision

Crystal structure of the sporulation histidine kinase inhibitor Sda from Bacillus subtilis

PDB ID 3fyr

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