3glt

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[[Image:3glt.png|left|200px]]
 
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{{STRUCTURE_3glt| PDB=3glt | SCENE= }}
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==Crystal Structure of Human SIRT3 with ADPR bound to the AceCS2 peptide containing a thioacetyl lysine==
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<StructureSection load='3glt' size='340' side='right'caption='[[3glt]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3glt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GLT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GLT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=FZN:(2S)-2-AMINO-6-{[(1Z)-1-{[(2R,3R,4S,5R)-5-({[(R)-{[(R)-{[(2R,3S,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3,4-DIHYDROXYTETRAHYDROFURAN-2-YL]METHOXY}(HYDROXY)PHOSPHORYL]OXY}(HYDROXY)PHOSPHORYL]OXY}METHYL)-3,4-DIHYDROXYTETRAHYDROFURAN-2-YL]SULFANYL}ETHYLIDENE]AMINO}HEXANOIC+ACID'>FZN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3glt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3glt OCA], [https://pdbe.org/3glt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3glt RCSB], [https://www.ebi.ac.uk/pdbsum/3glt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3glt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SIR3_HUMAN SIR3_HUMAN] NAD-dependent protein deacetylase. Activates mitochondrial target proteins, including ACSS1, IDH2 and GDH by deacetylating key lysine residues. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.<ref>PMID:16788062</ref> <ref>PMID:18680753</ref> <ref>PMID:18794531</ref> <ref>PMID:19535340</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gl/3glt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3glt ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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SIRT3 is a major mitochondrial NAD(+)-dependent protein deacetylase playing important roles in regulating mitochondrial metabolism and energy production and has been linked to the beneficial effects of exercise and caloric restriction. SIRT3 is emerging as a potential therapeutic target to treat metabolic and neurological diseases. We report the first sets of crystal structures of human SIRT3, an apo-structure with no substrate, a structure with a peptide containing acetyl lysine of its natural substrate acetyl-CoA synthetase 2, a reaction intermediate structure trapped by a thioacetyl peptide, and a structure with the dethioacetylated peptide bound. These structures provide insights into the conformational changes induced by the two substrates required for the reaction, the acetylated substrate peptide and NAD(+). In addition, the binding study by isothermal titration calorimetry suggests that the acetylated peptide is the first substrate to bind to SIRT3, before NAD(+). These structures and biophysical studies provide key insight into the structural and functional relationship of the SIRT3 deacetylation activity.
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===Crystal Structure of Human SIRT3 with ADPR bound to the AceCS2 peptide containing a thioacetyl lysine===
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Crystal structures of human SIRT3 displaying substrate-induced conformational changes.,Jin L, Wei W, Jiang Y, Peng H, Cai J, Mao C, Dai H, Choy W, Bemis JE, Jirousek MR, Milne JC, Westphal CH, Perni RB J Biol Chem. 2009 Sep 4;284(36):24394-405. Epub 2009 Jun 16. PMID:19535340<ref>PMID:19535340</ref>
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{{ABSTRACT_PUBMED_19535340}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3glt" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[3glt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GLT OCA].
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*[[Histone deacetylase 3D structures|Histone deacetylase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019535340</ref><references group="xtra"/>
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__TOC__
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[[Category: Acetate--CoA ligase]]
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Bemis, J E.]]
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[[Category: Large Structures]]
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[[Category: Cai, J.]]
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[[Category: Bemis JE]]
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[[Category: Dai, H.]]
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[[Category: Cai J]]
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[[Category: Jiang, Y.]]
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[[Category: Dai H]]
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[[Category: Jin, L.]]
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[[Category: Jiang Y]]
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[[Category: Jirousek, M R.]]
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[[Category: Jin L]]
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[[Category: Mao, C.]]
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[[Category: Jirousek MR]]
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[[Category: Milne, J C.]]
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[[Category: Mao C]]
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[[Category: Peng, H.]]
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[[Category: Milne JC]]
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[[Category: Perni, R B.]]
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[[Category: Peng H]]
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[[Category: Wei, W.]]
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[[Category: Perni RB]]
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[[Category: Westphal, C H.]]
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[[Category: Wei W]]
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[[Category: Hydrolase]]
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[[Category: Westphal CH]]
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[[Category: Hydrolase-hydrolase regulator complex]]
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[[Category: Intermediate trapped structure]]
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[[Category: Ligase]]
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[[Category: Metal-binding]]
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[[Category: Mitochondrion]]
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[[Category: Nad]]
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[[Category: Nad dependent deacetylase]]
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[[Category: Sirtuin]]
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[[Category: Thioacetyl peptide]]
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[[Category: Transit peptide]]
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Current revision

Crystal Structure of Human SIRT3 with ADPR bound to the AceCS2 peptide containing a thioacetyl lysine

PDB ID 3glt

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