3gx3

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[[Image:3gx3.png|left|200px]]
 
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{{STRUCTURE_3gx3| PDB=3gx3 | SCENE= }}
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==Crystal structure of the T. tengcongensis SAM-I riboswitch variant U34C/A94G bound with SAH==
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<StructureSection load='3gx3' size='340' side='right'caption='[[3gx3]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3gx3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GX3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GX3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gx3 OCA], [https://pdbe.org/3gx3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gx3 RCSB], [https://www.ebi.ac.uk/pdbsum/3gx3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gx3 ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The SAM-I riboswitch is a cis-acting element of genetic control found in bacterial mRNAs that specifically binds S-adenosylmethionine (SAM). We previously determined the 2.9-A X-ray crystal structure of the effector-binding domain of this RNA element, revealing details of RNA-ligand recognition. To improve this structure, variations were made to the RNA sequence to alter lattice contacts, resulting in a 0.5-A improvement in crystallographic resolution and allowing for a more accurate refinement of the crystallographic model. The basis for SAM specificity was addressed by a structural analysis of the RNA complexed to S-adenosylhomocysteine (SAH) and sinefungin and by measuring the affinity of SAM and SAH for a series of mutants using isothermal titration calorimetry. These data illustrate the importance of two universally conserved base pairs in the RNA that form electrostatic interactions with the positively charged sulfonium group of SAM, thereby providing a basis for discrimination between SAM and SAH.
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===Crystal structure of the T. tengcongensis SAM-I riboswitch variant U34C/A94G bound with SAH===
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Discrimination between closely related cellular metabolites by the SAM-I riboswitch.,Montange RK, Mondragon E, van Tyne D, Garst AD, Ceres P, Batey RT J Mol Biol. 2010 Feb 26;396(3):761-72. Epub 2009 Dec 16. PMID:20006621<ref>PMID:20006621</ref>
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{{ABSTRACT_PUBMED_20006621}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3gx3" style="background-color:#fffaf0;"></div>
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[[3gx3]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GX3 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:020006621</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Batey, R T.]]
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[[Category: Caldanaerobacter subterraneus subsp. tengcongensis]]
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[[Category: Montange, R K.]]
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[[Category: Large Structures]]
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[[Category: Four-way junction]]
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[[Category: Batey RT]]
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[[Category: Kink-turn]]
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[[Category: Montange RK]]
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[[Category: Pseudoknot]]
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[[Category: Riboswitch]]
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[[Category: Rna]]
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Current revision

Crystal structure of the T. tengcongensis SAM-I riboswitch variant U34C/A94G bound with SAH

PDB ID 3gx3

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