3vro

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[[Image:3vro.jpg|left|200px]]
 
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{{STRUCTURE_3vro| PDB=3vro | SCENE= }}
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==Crystal structure of the tyrosine kinase binding domain of Cbl-c in complex with phospho-Src peptide==
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<StructureSection load='3vro' size='340' side='right'caption='[[3vro]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3vro]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VRO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vro OCA], [https://pdbe.org/3vro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vro RCSB], [https://www.ebi.ac.uk/pdbsum/3vro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vro ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CBLC_HUMAN CBLC_HUMAN] Regulator of EGFR mediated signal transduction.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Through their ubiquitin ligase activity, Cbl-family proteins suppress signalling mediated by protein-tyrosine kinases (PTKs), but can also function as adaptor proteins to positively regulate signalling. The tyrosine kinase binding (TKB) domain of this family is critical for binding with tyrosine-phosphorylated target proteins. Here, we analysed the crystal structure of the TKB domain of Cbl-c/Cbl-3 (Cbl-c TKB), which is a distinct member of the mammalian Cbl-family. In comparison with Cbl TKB, Cbl-c TKB showed restricted structural flexibility upon phosphopeptide binding. A mutation in Cbl-c TKB augmenting this flexibility enhanced its binding to target phosphoproteins. These results suggest that proteins, post-translational modifications or mutations that alter structural flexibility of the TKB domain of Cbl-family proteins could regulate their binding to target phosphoproteins and thereby, affect PTK-mediated signalling.
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===Crystal structure of the tyrosine kinase binding domain of Cbl-c in complex with phospho-Src peptide===
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Structural flexibility regulates phosphopeptide-binding activity of the tyrosine kinase binding domain of Cbl-c.,Takeshita K, Tezuka T, Isozaki Y, Yamashita E, Suzuki M, Kim M, Yamanashi Y, Yamamoto T, Nakagawa A J Biochem. 2012 Nov;152(5):487-95. doi: 10.1093/jb/mvs085. Epub 2012 Aug 9. PMID:22888118<ref>PMID:22888118</ref>
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{{ABSTRACT_PUBMED_22888118}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3vro" style="background-color:#fffaf0;"></div>
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[[3vro]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VRO OCA].
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Non-specific protein-tyrosine kinase]]
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[[Category: Large Structures]]
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[[Category: Isozaki, Y.]]
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[[Category: Isozaki Y]]
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[[Category: Nakagawa, A.]]
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[[Category: Nakagawa A]]
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[[Category: Suzuki, M.]]
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[[Category: Suzuki M]]
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[[Category: Takeshita, K.]]
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[[Category: Takeshita K]]
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[[Category: Tezuka, T.]]
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[[Category: Tezuka T]]
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[[Category: Yamamoto, T.]]
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[[Category: Yamamoto T]]
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[[Category: Yamanashi, Y.]]
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[[Category: Yamanashi Y]]
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[[Category: Yamashita, E.]]
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[[Category: Yamashita E]]
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[[Category: Calcium-binding ef hand]]
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[[Category: Divergent sh2 domain]]
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[[Category: Protein binding-transferase complex]]
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[[Category: Ptb domain]]
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[[Category: Regulator of egfr mediated signal transduction]]
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[[Category: Ubiquitously expressed]]
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Current revision

Crystal structure of the tyrosine kinase binding domain of Cbl-c in complex with phospho-Src peptide

PDB ID 3vro

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