4h4k
From Proteopedia
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| - | [[Image:4h4k.jpg|left|200px]] | ||
| - | + | ==Structure of the Cmr2-Cmr3 subcomplex of the Cmr RNA-silencing complex== | |
| + | <StructureSection load='4h4k' size='340' side='right'caption='[[4h4k]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4h4k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H4K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H4K FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.804Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h4k OCA], [https://pdbe.org/4h4k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h4k RCSB], [https://www.ebi.ac.uk/pdbsum/4h4k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h4k ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CMR3_PYRFU CMR3_PYRFU] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), formerly called psiRNA (prokaryotic silencing) in this organism. Part of the Cmr ribonucleoprotein complex which has divalent cation-dependent endoribonuclease activity specific for ssRNA complementary to the crRNA, generating 5' hydroxy- and 3' phosphate or 2'-3' cyclic phosphate termini. It is not known which subunit has endoribonuclease activity. Cmr complex does not cleave ssDNA complementary to the crRNA. Cleavage of invading RNA is guided by the crRNA; substrate cleavage occurs a fixed distance (14 nt) from the 3' end of the crRNA. In vitro reconstitution shows Cmr1-2 and Cmr5 are not necessary for cleavage. | ||
| - | == | + | ==See Also== |
| - | + | *[[Endonuclease 3D structures|Endonuclease 3D structures]] | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | + | [[Category: Large Structures]] | |
| - | [[ | + | [[Category: Pyrococcus furiosus DSM 3638]] |
| - | [[Category: Pyrococcus furiosus | + | [[Category: Cocozaki AI]] |
| - | [[Category: Cocozaki | + | [[Category: Li H]] |
| - | [[Category: Li | + | [[Category: Ramia NF]] |
| - | [[Category: Ramia | + | [[Category: Shao Y]] |
| - | [[Category: Shao | + | [[Category: Terns MP]] |
| - | [[Category: Terns | + | [[Category: Terns RM]] |
| - | [[Category: Terns | + | |
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Current revision
Structure of the Cmr2-Cmr3 subcomplex of the Cmr RNA-silencing complex
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