3hda

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:20, 6 September 2023) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:3hda.png|left|200px]]
 
-
{{STRUCTURE_3hda| PDB=3hda | SCENE= }}
+
==PrtC methionine mutants: M226A_DESY==
 +
<StructureSection load='3hda' size='340' side='right'caption='[[3hda]], [[Resolution|resolution]] 2.13&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3hda]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_chrysanthemi Dickeya chrysanthemi] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HDA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HDA FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.131&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hda FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hda OCA], [https://pdbe.org/3hda PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hda RCSB], [https://www.ebi.ac.uk/pdbsum/3hda PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hda ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PRTC_DICCH PRTC_DICCH]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hd/3hda_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hda ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Abstract The metzincins constitute a subclan of metalloproteases possessing a HEXXHXXGXXH/D zinc-binding consensus sequence where the three histidines are zinc ligands and the glutamic acid the catalytic base. A completely conserved methionine is located downstream of this motif. Families of the metzincin clan comprise, besides others, astacins, ADAMS proteases, matrix metalloproteases and serralysins. The latter are extracellular 50 kDa proteases secreted by Gram-negative bacteria via a type I secretion system. While there is a large body of structural and biochemical information available, the function of the conserved methionine has not been convincingly clarified yet. Here we present the crystal structures of a number of mutants of the serralysin member protease C with the conserved methionine being replaced by Ile, Ala and His. Together with our former report on the leucine and cysteine mutants, we demonstrate here that replacement of the methionine side chain results in an increasing distortion of the zinc-binding geometry, especially pronounced in the chi(2) angles of the first and third histidine of the consensus sequence. This is correlated with an increasing loss of proteolytic activity and a sharp increase of flexibility of large segments of the polypeptide chain.
-
===PrtC methionine mutants: M226A_DESY===
+
Metzincin's canonical methionine is responsible for the structural integrity of the zinc-binding site.,Oberholzer AE, Bumann M, Hege T, Russo S, Baumann U Biol Chem. 2009 Jun 27. PMID:19558324<ref>PMID:19558324</ref>
-
{{ABSTRACT_PUBMED_19558324}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 3hda" style="background-color:#fffaf0;"></div>
-
[[3hda]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HDA OCA].
+
== References ==
-
 
+
<references/>
-
==Reference==
+
__TOC__
-
<ref group="xtra">PMID:019558324</ref><references group="xtra"/>
+
</StructureSection>
-
[[Category: Erwinia chrysanthemi]]
+
[[Category: Dickeya chrysanthemi]]
-
[[Category: Baumann, U.]]
+
[[Category: Large Structures]]
-
[[Category: Bumann, M.]]
+
[[Category: Synthetic construct]]
-
[[Category: Hege, T.]]
+
[[Category: Baumann U]]
-
[[Category: Oberholzer, A E.]]
+
[[Category: Bumann M]]
-
[[Category: Russo, S.]]
+
[[Category: Hege T]]
-
[[Category: Beta roll]]
+
[[Category: Oberholzer AE]]
-
[[Category: Hydrolase]]
+
[[Category: Russo S]]
-
[[Category: Met-turn]]
+
-
[[Category: Metal-binding]]
+
-
[[Category: Metalloprotease]]
+
-
[[Category: Metzincin]]
+
-
[[Category: Protease]]
+
-
[[Category: Secreted]]
+
-
[[Category: Zymogen]]
+

Current revision

PrtC methionine mutants: M226A_DESY

PDB ID 3hda

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3hda"
Personal tools