3hhf

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[[Image:3hhf.png|left|200px]]
 
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{{STRUCTURE_3hhf| PDB=3hhf | SCENE= }}
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==Structure of CrgA regulatory domain, a LysR-type transcriptional regulator from Neisseria meningitidis.==
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<StructureSection load='3hhf' size='340' side='right'caption='[[3hhf]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3hhf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_meningitidis_serogroup_B Neisseria meningitidis serogroup B]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HHF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hhf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hhf OCA], [https://pdbe.org/3hhf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hhf RCSB], [https://www.ebi.ac.uk/pdbsum/3hhf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hhf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CRGA_NEIMB CRGA_NEIMB] Regulatory protein that activates transcription of mdaB, encoding a NADPH:quinone oxidoreductase, and represses its own transcription (PubMed:15866928). Under the same experimental conditions, no regulation of transcription of pilus and capsule genes is detected (PubMed:15866928).<ref>PMID:15866928</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/3hhf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hhf ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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LysR-type transcriptional regulators (LTTRs) form the largest family of bacterial regulators acting as both auto-repressors and activators of target promoters, controlling operons involved in a wide variety of cellular processes. The LTTR, CrgA, from the human pathogen Neisseria meningitidis, is upregulated during bacterial-host cell contact. Here, we report the crystal structures of both regulatory domain and full-length CrgA, the first of a novel subclass of LTTRs that form octameric rings. Non-denaturing mass spectrometry analysis and analytical ultracentrifugation established that the octameric form of CrgA is the predominant species in solution in both the presence and absence of an oligonucleotide encompassing the CrgA-binding sequence. Furthermore, analysis of the isolated CrgA-DNA complex by mass spectrometry showed stabilization of a double octamer species upon DNA binding. Based on the observed structure and the mass spectrometry findings, a model is proposed in which a hexadecameric array of two CrgA oligomers binds to its DNA target site.
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===Structure of CrgA regulatory domain, a LysR-type transcriptional regulator from Neisseria meningitidis.===
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The structure of CrgA from Neisseria meningitidis reveals a new octameric assembly state for LysR transcriptional regulators.,Sainsbury S, Lane LA, Ren J, Gilbert RJ, Saunders NJ, Robinson CV, Stuart DI, Owens RJ Nucleic Acids Res. 2009 Aug;37(14):4545-58. Epub 2009 May 27. PMID:19474343<ref>PMID:19474343</ref>
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{{ABSTRACT_PUBMED_19474343}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3hhf" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[3hhf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Neisseria_meningitidis_serogroup_b Neisseria meningitidis serogroup b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HHF OCA].
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*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019474343</ref><references group="xtra"/>
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__TOC__
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[[Category: Neisseria meningitidis serogroup b]]
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</StructureSection>
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[[Category: OPPF, Oxford Protein Production Facility.]]
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[[Category: Large Structures]]
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[[Category: Owens, R J.]]
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[[Category: Neisseria meningitidis serogroup B]]
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[[Category: Ren, J.]]
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[[Category: Owens RJ]]
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[[Category: Sainsbury, S.]]
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[[Category: Ren J]]
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[[Category: Stuart, D I.]]
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[[Category: Sainsbury S]]
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[[Category: Dna-binding]]
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[[Category: Stuart DI]]
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[[Category: Lysr]]
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[[Category: Neisseria meningitidi]]
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[[Category: Oppf]]
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[[Category: Oxford protein production facility]]
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[[Category: Structural genomic]]
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[[Category: Transcription]]
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[[Category: Transcription factor]]
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[[Category: Transcription regulation]]
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[[Category: Transcription regulator]]
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Current revision

Structure of CrgA regulatory domain, a LysR-type transcriptional regulator from Neisseria meningitidis.

PDB ID 3hhf

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