2w7n

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:08, 9 May 2024) (edit) (undo)
 
(6 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2w7n.png|left|200px]]
 
-
{{STRUCTURE_2w7n| PDB=2w7n | SCENE= }}
+
==Crystal Structure of KorA Bound to Operator DNA: Insight into Repressor Cooperation in RP4 Gene Regulation==
 +
<StructureSection load='2w7n' size='340' side='right'caption='[[2w7n]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2w7n]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W7N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W7N FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BRU:5-BROMO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>BRU</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w7n OCA], [https://pdbe.org/2w7n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w7n RCSB], [https://www.ebi.ac.uk/pdbsum/2w7n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w7n ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/KORA2_ECOLX KORA2_ECOLX] In conjunction with KorB, inhibits the transcription of kilA, trfA and korAB operons. In conjunction with KorC is responsible for the negative control of kilC and kilE operons.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w7/2w7n_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w7n ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
KorA is a global repressor in RP4 which regulates cooperatively the expression of plasmid genes whose products are involved in replication, conjugative transfer and stable inheritance. The structure of KorA bound to an 18-bp DNA duplex that contains the symmetric operator sequence and incorporates 5-bromo-deoxyuridine nucleosides has been determined by multiple-wavelength anomalous diffraction phasing at 1.96-A resolution. KorA is present as a symmetric dimer and contacts DNA via a helix-turn-helix motif. Each half-site of the symmetric operator DNA binds one copy of the protein in the major groove. As confirmed by mutagenesis, recognition specificity is based on two KorA side chains forming hydrogen bonds to four bases within each operator half-site. KorA has a unique dimerization module shared by the RP4 proteins TrbA and KlcB. We propose that these proteins cooperate with the global RP4 repressor KorB in a similar manner via this dimerization module and thus regulate RP4 inheritance.
-
===CRYSTAL STRUCTURE OF KORA BOUND TO OPERATOR DNA: INSIGHT INTO REPRESSOR COOPERATION IN RP4 GENE REGULATION===
+
Crystal structure of KorA bound to operator DNA: insight into repressor cooperation in RP4 gene regulation.,Konig B, Muller JJ, Lanka E, Heinemann U Nucleic Acids Res. 2009 Apr;37(6):1915-24. Epub 2009 Feb 3. PMID:19190096<ref>PMID:19190096</ref>
-
{{ABSTRACT_PUBMED_19190096}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 2w7n" style="background-color:#fffaf0;"></div>
-
[[2w7n]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W7N OCA].
+
== References ==
-
 
+
<references/>
-
==Reference==
+
__TOC__
-
<ref group="xtra">PMID:019190096</ref><references group="xtra"/>
+
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Heinemann, U.]]
+
[[Category: Large Structures]]
-
[[Category: Koenig, B.]]
+
[[Category: Heinemann U]]
-
[[Category: Lanka, E.]]
+
[[Category: Koenig B]]
-
[[Category: Mueller, J J.]]
+
[[Category: Lanka E]]
-
[[Category: Dna-binding]]
+
[[Category: Mueller JJ]]
-
[[Category: Helix-turn-helix motif]]
+
-
[[Category: Incp]]
+
-
[[Category: Protein-dna complex]]
+
-
[[Category: Repressor]]
+
-
[[Category: Transcription factor]]
+
-
[[Category: Transcription regulation]]
+
-
[[Category: Transcription-dna complex]]
+
-
[[Category: Transcription/dna]]
+

Current revision

Crystal Structure of KorA Bound to Operator DNA: Insight into Repressor Cooperation in RP4 Gene Regulation

PDB ID 2w7n

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2w7n"
Personal tools