Galactose oxidase

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{{STRUCTURE_1gog| PDB=1gog | SIZE=400| SCENE= |right|CAPTION=Galactose oxidase with Cu+2 and Na+ ions, [[1gog]] }}
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<StructureSection load='1gog' size='350' side='right' scene='48/486468/Cv/1' caption='Galactose oxidase with Cu+2 (orange) and Na+ ions (purple), [[1gog]]'>
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== Function ==
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'''Galactose oxidase''' (GAO) catalyzes the oxidation of D-galactose using O2 to D-galacto-hexodialdose and hydrogen peroxide. GAO uses copper and active site post-translational cross-linking of Cys228 and Tyr272 via a thioether bond for activity. GAO catalytic motif is a free radical-coupled copper.
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'''Galactose oxidase''' (GAO) catalyzes the oxidation of D-galactose using O2 to D-galacto-hexodialdose and hydrogen peroxide. GAO catalytic motif is a free radical-coupled copper<ref>PMID:7929198</ref>.
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== Structural highlights ==
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GAO uses <scene name='48/486468/Cv/6'>copper and active site</scene> post-translational <scene name='48/486468/Cv/7'>cross-linking of Cys228 and Tyr272 via a thioester bond</scene> for activity<ref>PMID:2002850</ref>. Water molecules shown as red spheres. <scene name='48/486468/Cv/8'>Na coordination site</scene>.
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</StructureSection>
==3D structures of galactose oxidase==
==3D structures of galactose oxidase==
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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[[1gof]], [[1gog]], [[1goh]] – GAO – ''Hypomyces rosellus''<br />
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[[1gof]], [[1gog]], [[1goh]] – GAO + Cu – ''Hypomyces rosellus''<br />
[[1k3i]] – FuGAO precursor – ''Fusarium''<br />
[[1k3i]] – FuGAO precursor – ''Fusarium''<br />
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[[1t2x]] – FuGAO (mutant)<br />
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[[8tx5]], [[8tx6]] - FuGAO + Cu
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[[2eib]], [[2eic]], [[2eid]], [[2wq8]] - GzGAO (mutant) – ''Gibberella zeae''<br />
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[[1t2x]] – FuGAO (mutant) + Cu<br />
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[[2vz3]] - GzGAO + Cu – ''Gibberella zeae''<br />
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[[2eib]], [[2eic]], [[2eid]], [[2wq8]] - GzGAO (mutant) + Cu<br />
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[[6xlt]] – GzGAO + Cu <br />
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[[6xlr]], [[6xls]] – GzGAO + Cu + modified Tyr272<br />
[[2eie]] – GzGAO + N3<br />
[[2eie]] – GzGAO + N3<br />
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[[2jkx]] – GzGAO no Cu<br />
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[[2jkx]] – GzGAO <br />
[[2vz1]] – GzGAO premature<br />
[[2vz1]] – GzGAO premature<br />
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[[2vz3]] - GzGAO
 
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== References ==
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<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Galactose oxidase with Cu+2 (orange) and Na+ ions (purple), 1gog

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3D structures of galactose oxidase

Updated on 07-July-2025

1gof, 1gog, 1goh – GAO + Cu – Hypomyces rosellus
1k3i – FuGAO precursor – Fusarium
8tx5, 8tx6 - FuGAO + Cu 1t2x – FuGAO (mutant) + Cu
2vz3 - GzGAO + Cu – Gibberella zeae
2eib, 2eic, 2eid, 2wq8 - GzGAO (mutant) + Cu
6xlt – GzGAO + Cu
6xlr, 6xls – GzGAO + Cu + modified Tyr272
2eie – GzGAO + N3
2jkx – GzGAO
2vz1 – GzGAO premature

References

  1. ↑ Baron AJ, Stevens C, Wilmot C, Seneviratne KD, Blakeley V, Dooley DM, Phillips SE, Knowles PF, McPherson MJ. Structure and mechanism of galactose oxidase. The free radical site. J Biol Chem. 1994 Oct 7;269(40):25095-105. PMID:7929198
  2. ↑ Ito N, Phillips SE, Stevens C, Ogel ZB, McPherson MJ, Keen JN, Yadav KD, Knowles PF. Novel thioether bond revealed by a 1.7 A crystal structure of galactose oxidase. Nature. 1991 Mar 7;350(6313):87-90. PMID:2002850 doi:http://dx.doi.org/10.1038/350087a0

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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