Homocitrate synthase
From Proteopedia
(Difference between revisions)
| (9 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | + | <StructureSection load='3ivt' size='400' side='right' scene='46/465446/Cv/1' caption='Fission yeast homocitrate synthase Lys4 dimer complex with 2-oxoglutarate, Na+ (purple) and Zn+2 (grey) ions [[3ivt]]'> | |
| - | '''Homocitrate synthase''' (HS) participates in lysine biosynthesis and pyruvate metabolism. HS catalyzes the conversion of acetyl-CoA + H2O + 2-oxoglutarate to 2-hydroxybutane-1,2,4-tricarboxylate + CoA. | + | == Function == |
| - | + | '''Homocitrate synthase''' (HS) participates in lysine biosynthesis and pyruvate metabolism. HS catalyzes the conversion of acetyl-CoA + H2O + 2-oxoglutarate to 2-hydroxybutane-1,2,4-tricarboxylate + CoA<ref>PMID:5836514</ref>. | |
| - | + | ||
| + | == Structural highlights == | ||
| + | The HS <scene name='46/465446/Cv/7'>active site is located in the interior of the N-terminal TIM-barrel domain</scene>. | ||
| + | *<scene name='46/465446/Cv/8'>Active site</scene>. | ||
| + | *The <scene name='46/465446/Cv/9'>metal ion is octahedrally coordinated to the protein and to the 2-oxoglutarate</scene><ref>PMID:19776021</ref>. Water molecules shown as red spheres. | ||
| + | </StructureSection> | ||
== 3D Structures of homocitrate synthase == | == 3D Structures of homocitrate synthase == | ||
| Line 10: | Line 14: | ||
[[3ivs]] – fyHS Lys4 – fission yeast<br /> | [[3ivs]] – fyHS Lys4 – fission yeast<br /> | ||
| - | [[3ivt]], [[3ivu]] - fyHS Lys4 + 2- | + | [[3ivt]], [[3ivu]] - fyHS Lys4 + 2-oxoglutarate<br /> |
[[3mi3]] - fyHS Lys4 + lysine<br /> | [[3mi3]] - fyHS Lys4 + lysine<br /> | ||
[[2ztj]] – TtHS + α-ketoglutarate – ''Thermus thermophilus''<br /> | [[2ztj]] – TtHS + α-ketoglutarate – ''Thermus thermophilus''<br /> | ||
[[2zyf]] - TtHS + α-ketoglutarate + Mg<br /> | [[2zyf]] - TtHS + α-ketoglutarate + Mg<br /> | ||
[[2ztk]] – TtHS + homocitrate<br /> | [[2ztk]] – TtHS + homocitrate<br /> | ||
| - | [[3a9i]] – TtHS + lysine | + | [[3a9i]] – TtHS + lysine<br /> |
| - | + | [[6ktq]] - HS + -ketoglutarate + CoA – ''Sulfolobus acidocaldarius''<br /> | |
| + | == References == | ||
| + | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Current revision
| |||||||||||
3D Structures of homocitrate synthase
Updated on 27-September-2021
3ivs – fyHS Lys4 – fission yeast
3ivt, 3ivu - fyHS Lys4 + 2-oxoglutarate
3mi3 - fyHS Lys4 + lysine
2ztj – TtHS + α-ketoglutarate – Thermus thermophilus
2zyf - TtHS + α-ketoglutarate + Mg
2ztk – TtHS + homocitrate
3a9i – TtHS + lysine
6ktq - HS + -ketoglutarate + CoA – Sulfolobus acidocaldarius
References
- ↑ Strassman M, Ceci LN. Enzymatic formation of homocitric acid, an intermediate in lysine biosynthesis. Biochem Biophys Res Commun. 1964;14:262-7. PMID:5836514
- ↑ Bulfer SL, Scott EM, Couture JF, Pillus L, Trievel RC. Crystal structure and functional analysis of homocitrate synthase, an essential enzyme in lysine biosynthesis. J Biol Chem. 2009 Dec 18;284(51):35769-80. Epub . PMID:19776021 doi:10.1074/jbc.M109.046821
