2b3j

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[[Image:2b3j.gif|left|200px]]<br /><applet load="2b3j" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2b3j, resolution 2.00&Aring;" />
 
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'''Crystal Structure of Staphylococcus aureus tRNA Adenosine Deaminase, TadA, in Complex with RNA'''<br />
 
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==Overview==
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==Crystal Structure of Staphylococcus aureus tRNA Adenosine Deaminase, TadA, in Complex with RNA==
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Bacterial tRNA adenosine deaminases (TadAs) catalyze the hydrolytic deamination of adenosine to inosine at the wobble position of tRNA(Arg2), a process that enables this single tRNA to recognize three different arginine codons in mRNA. In addition, inosine is also introduced at the wobble position of multiple eukaryotic tRNAs. The genes encoding these deaminases are essential in bacteria and yeast, demonstrating the importance of their biological activity. Here we report the crystallization and structure determination to 2.0 A of Staphylococcus aureus TadA bound to the anticodon stem-loop of tRNA(Arg2) bearing nebularine, a non-hydrolyzable adenosine analog, at the wobble position. The cocrystal structure reveals the basis for both sequence and structure specificity in the interactions of TadA with RNA, and it additionally provides insight into the active site architecture that promotes efficient hydrolytic deamination.
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<StructureSection load='2b3j' size='340' side='right'caption='[[2b3j]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2b3j]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B3J FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P5P:PURINE+RIBOSIDE-5-MONOPHOSPHATE'>P5P</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3j OCA], [https://pdbe.org/2b3j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b3j RCSB], [https://www.ebi.ac.uk/pdbsum/2b3j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b3j ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TADA_STAAM TADA_STAAM] Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2).[HAMAP-Rule:MF_00972]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b3/2b3j_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b3j ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2B3J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3J OCA].
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*[[Adenosine deaminase 3D structures|Adenosine deaminase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of Staphylococcus aureus tRNA adenosine deaminase TadA in complex with RNA., Losey HC, Ruthenburg AJ, Verdine GL, Nat Struct Mol Biol. 2006 Feb;13(2):153-9. Epub 2006 Jan 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16415880 16415880]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Staphylococcus aureus subsp. aureus Mu50]]
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[[Category: Staphylococcus aureus subsp. aureus mu50]]
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[[Category: Losey HC]]
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[[Category: Losey, H C.]]
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[[Category: Ruthenburg AJ]]
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[[Category: Ruthenburg, A J.]]
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[[Category: Verdine GL]]
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[[Category: Verdine, G L.]]
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[[Category: GOL]]
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[[Category: ZN]]
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[[Category: mixed alpha-beta]]
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[[Category: protein-rna complex]]
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[[Category: rna stem-loop]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:33:46 2008''
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Current revision

Crystal Structure of Staphylococcus aureus tRNA Adenosine Deaminase, TadA, in Complex with RNA

PDB ID 2b3j

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