3oto

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{{Large structure}}
 
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{{STRUCTURE_3oto| PDB=3oto | SCENE= }}
 
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===Crystal Structure of the 30S ribosomal subunit from a KsgA mutant of Thermus thermophilus (HB8)===
 
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{{ABSTRACT_PUBMED_20962038}}
 
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==About this Structure==
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==Crystal Structure of the 30S ribosomal subunit from a KsgA mutant of Thermus thermophilus (HB8)==
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[[3oto]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTO OCA].
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<StructureSection load='3oto' size='340' side='right'caption='[[3oto]], [[Resolution|resolution]] 3.69&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3oto]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OTO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.69&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oto OCA], [https://pdbe.org/3oto PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oto RCSB], [https://www.ebi.ac.uk/pdbsum/3oto PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oto ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RS2_THET8 RS2_THET8] Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ot/3oto_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3oto ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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All organisms incorporate post-transcriptional modifications into ribosomal RNA, influencing ribosome assembly and function in ways that are poorly understood. The most highly conserved modification is the dimethylation of two adenosines near the 3' end of the small subunit rRNA. Lack of these methylations due to deficiency in the KsgA methyltransferase stimulates translational errors during both the initiation and elongation phases of protein synthesis and confers resistance to the antibiotic kasugamycin. Here, we present the X-ray crystal structure of the Thermus thermophilus 30S ribosomal subunit lacking these dimethylations. Our data indicate that the KsgA-directed methylations facilitate structural rearrangements in order to establish a functionally optimum subunit conformation during the final stages of ribosome assembly.
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==See Also==
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Modification of 16S ribosomal RNA by the KsgA methyltransferase restructures the 30S subunit to optimize ribosome function.,Demirci H, Murphy F 4th, Belardinelli R, Kelley AC, Ramakrishnan V, Gregory ST, Dahlberg AE, Jogl G RNA. 2010 Oct 20. PMID:20962038<ref>PMID:20962038</ref>
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*[[Ribosomal protein S10|Ribosomal protein S10]]
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*[[Ribosomal protein S11|Ribosomal protein S11]]
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*[[Ribosomal protein S12|Ribosomal protein S12]]
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*[[Ribosomal protein S13|Ribosomal protein S13]]
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*[[Ribosomal protein S14|Ribosomal protein S14]]
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*[[Ribosomal protein S15|Ribosomal protein S15]]
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*[[Ribosomal protein S16|Ribosomal protein S16]]
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*[[Ribosomal protein S17|Ribosomal protein S17]]
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*[[Ribosomal protein S18|Ribosomal protein S18]]
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*[[Ribosomal protein S19|Ribosomal protein S19]]
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*[[Ribosomal protein S2|Ribosomal protein S2]]
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*[[Ribosomal protein S20|Ribosomal protein S20]]
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*[[Ribosomal protein S3|Ribosomal protein S3]]
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*[[Ribosomal protein S4|Ribosomal protein S4]]
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*[[Ribosomal protein S5|Ribosomal protein S5]]
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*[[Ribosomal protein S6|Ribosomal protein S6]]
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*[[Ribosomal protein S7|Ribosomal protein S7]]
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*[[Ribosomal protein S8|Ribosomal protein S8]]
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*[[Ribosomal protein S9|Ribosomal protein S9]]
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*[[Ribosomal protein THX|Ribosomal protein THX]]
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:020962038</ref><references group="xtra"/>
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</div>
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[[Category: Thermus thermophilus]]
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<div class="pdbe-citations 3oto" style="background-color:#fffaf0;"></div>
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[[Category: Belardinelli, R.]]
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[[Category: Dahlberg, A E.]]
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==See Also==
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[[Category: Demirci, H.]]
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*[[Ribosomal protein THX 3D structures|Ribosomal protein THX 3D structures]]
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[[Category: Gregory, S T.]]
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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[[Category: IV, F Murphy.]]
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== References ==
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[[Category: Jogl, G.]]
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<references/>
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[[Category: Kelley, A C.]]
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__TOC__
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[[Category: Ramakrishnan, V.]]
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</StructureSection>
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[[Category: 30s ribosomal subunit]]
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[[Category: Large Structures]]
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[[Category: Antibiotic resistance]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Decoding]]
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[[Category: Belardinelli R]]
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[[Category: Decoding of genetic code]]
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[[Category: Dahlberg AE]]
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[[Category: Ksga knock-out]]
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[[Category: Demirci H]]
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[[Category: Mrna]]
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[[Category: Gregory ST]]
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[[Category: Post transcriptional rrna modification]]
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[[Category: Jogl G]]
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[[Category: Ribosome]]
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[[Category: Kelley AC]]
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[[Category: Trna]]
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[[Category: Murphy IV F]]
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[[Category: Ramakrishnan V]]

Current revision

Crystal Structure of the 30S ribosomal subunit from a KsgA mutant of Thermus thermophilus (HB8)

PDB ID 3oto

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