2wb1

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{{Large structure}}
 
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{{STRUCTURE_2wb1| PDB=2wb1 | SCENE= }}
 
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===THE COMPLETE STRUCTURE OF THE ARCHAEAL 13-SUBUNIT DNA-DIRECTED RNA POLYMERASE===
 
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{{ABSTRACT_PUBMED_19419240}}
 
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==About this Structure==
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==The complete structure of the archaeal 13-subunit DNA-directed RNA Polymerase==
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[[2wb1]] is a 26 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_shibatae Sulfolobus shibatae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WB1 OCA].
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<StructureSection load='2wb1' size='340' side='right'caption='[[2wb1]], [[Resolution|resolution]] 3.52&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2wb1]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_shibatae Saccharolobus shibatae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WB1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WB1 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.52&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wb1 OCA], [https://pdbe.org/2wb1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wb1 RCSB], [https://www.ebi.ac.uk/pdbsum/2wb1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wb1 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPO1N_SACSH RPO1N_SACSH] DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms the clamp head domain.[HAMAP-Rule:MF_00863]<ref>PMID:19419240</ref> <ref>PMID:21265742</ref> <ref>PMID:22848102</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wb/2wb1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wb1 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II but requires a reduced subset of general transcription factors for promoter-dependent initiation. To deepen our knowledge of cellular transcription, we have determined the structure of the 13-subunit DNA-directed RNAP from Sulfolobus shibatae at 3.35 A resolution. The structure contains the full complement of subunits, including RpoG/Rpb8 and the equivalent of the clamp-head and jaw domains of the eukaryotic Rpb1. Furthermore, we have identified subunit Rpo13, an RNAP component in the order Sulfolobales, which contains a helix-turn-helix motif that interacts with the RpoH/Rpb5 and RpoA'/Rpb1 subunits. Its location and topology suggest a role in the formation of the transcription bubble.
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==See Also==
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Evolution of Complex RNA Polymerases: The Complete Archaeal RNA Polymerase Structure.,Korkhin Y, Unligil UM, Littlefield O, Nelson PJ, Stuart DI, Sigler PB, Bell SD, Abrescia NG PLoS Biol. 2009 May 5;7(5):e102. PMID:19419240<ref>PMID:19419240</ref>
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*[[RNA polymerase|RNA polymerase]]
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:019419240</ref><references group="xtra"/>
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</div>
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[[Category: Sulfolobus shibatae]]
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<div class="pdbe-citations 2wb1" style="background-color:#fffaf0;"></div>
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[[Category: Abrescia, N G.A.]]
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[[Category: Bell, S D.]]
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==See Also==
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[[Category: Korkhin, Y.]]
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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[[Category: Littlefield, O.]]
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== References ==
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[[Category: Nelson, P J.]]
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<references/>
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[[Category: Sigler, P B.]]
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__TOC__
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[[Category: Stuart, D I.]]
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</StructureSection>
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[[Category: Unligil, U M.]]
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[[Category: Large Structures]]
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[[Category: Multi-subunit enzyme]]
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[[Category: Saccharolobus shibatae]]
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[[Category: Rna-polymerase]]
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[[Category: Abrescia NGA]]
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[[Category: Transcription]]
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[[Category: Bell SD]]
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[[Category: Korkhin Y]]
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[[Category: Littlefield O]]
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[[Category: Nelson PJ]]
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[[Category: Sigler PB]]
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[[Category: Stuart DI]]
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[[Category: Unligil UM]]

Current revision

The complete structure of the archaeal 13-subunit DNA-directed RNA Polymerase

PDB ID 2wb1

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