3nbu

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{{STRUCTURE_3nbu| PDB=3nbu | SCENE= }}
 
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===Crystal structure of pGI glucosephosphate isomerase===
 
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{{ABSTRACT_PUBMED_22393408}}
 
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==About this Structure==
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==Crystal structure of pGI glucosephosphate isomerase==
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[[3nbu]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NBU OCA].
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<StructureSection load='3nbu' size='340' side='right'caption='[[3nbu]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3nbu]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_DH5alpha Escherichia coli DH5alpha]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NBU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NBU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nbu OCA], [https://pdbe.org/3nbu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nbu RCSB], [https://www.ebi.ac.uk/pdbsum/3nbu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nbu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/G6PI_ECOLI G6PI_ECOLI]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Structural biology and structural genomics projects routinely rely on recombinantly expressed proteins, but many proteins and complexes are difficult to obtain by this approach. We investigated native source proteins for high-throughput protein crystallography applications. The Escherichia coli proteome was fractionated, purified, crystallized, and structurally characterized. Macro-scale fermentation and fractionation were used to subdivide the soluble proteome into 408 unique fractions of which 295 fractions yielded crystals in microfluidic crystallization chips. Of the 295 crystals, 152 were selected for optimization, diffraction screening, and data collection. Twenty-three structures were determined, four of which were novel. This study demonstrates the utility of native source proteins for high-throughput crystallography.
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==See Also==
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Macro-to-Micro Structural Proteomics: Native Source Proteins for High-Throughput Crystallization.,Totir M, Echols N, Nanao M, Gee CL, Moskaleva A, Gradia S, Iavarone AT, Berger JM, May AP, Zubieta C, Alber T PLoS One. 2012;7(2):e32498. Epub 2012 Feb 29. PMID:22393408<ref>PMID:22393408</ref>
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*[[Phosphoglucoisomerase|Phosphoglucoisomerase]]
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*[[Phosphoglucose isomerase|Phosphoglucose isomerase]]
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:022393408</ref><references group="xtra"/>
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</div>
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[[Category: Escherichia coli]]
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<div class="pdbe-citations 3nbu" style="background-color:#fffaf0;"></div>
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[[Category: Glucose-6-phosphate isomerase]]
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[[Category: Alber, T.]]
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==See Also==
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[[Category: Echols, N.]]
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*[[Phosphoglucose isomerase 3D structures|Phosphoglucose isomerase 3D structures]]
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[[Category: May, A.]]
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== References ==
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[[Category: Totir, M.]]
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<references/>
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[[Category: Zubieta, C.]]
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__TOC__
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[[Category: Isomerase]]
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Alber T]]
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[[Category: Echols N]]
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[[Category: May A]]
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[[Category: Totir M]]
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[[Category: Zubieta C]]

Current revision

Crystal structure of pGI glucosephosphate isomerase

PDB ID 3nbu

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