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2b7u

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[[Image:2b7u.gif|left|200px]]<br /><applet load="2b7u" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2b7u, resolution 1.60&Aring;" />
 
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'''Ribosome inactivating protein type 1 from Charybdis maritima AGG'''<br />
 
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==Overview==
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==Ribosome inactivating protein type 1 from Charybdis maritima AGG==
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A novel, type 1 ribosome-inactivating protein designated charybdin was isolated from bulbs of Charybdis maritima agg. The protein, consisting of a single polypeptide chain with a molecular mass of 29 kDa, inhibited translation in rabbit reticulocytes with an IC50 of 27.2 nm. Plant genomic DNA extracted from the bulb was amplified by PCR between primers based on the N-terminal and C-terminal sequence of the protein from dissolved crystals. The complete mature protein sequence was derived by partial DNA sequencing and terminal protein sequencing, and was confirmed by high-resolution crystal structure analysis. The protein contains Val at position 79 instead of the conserved Tyr residue of the ribosome-inactivating proteins known to date. To our knowledge, this is the first observation of a natural substitution of a catalytic residue at the active site of a natural ribosome-inactivating protein. This substitution in the active site may be responsible for the relatively low in vitro translation inhibitory effect compared with other ribosome-inactivating proteins. Single crystals were grown in the cold room from PEG6000 solutions. Diffraction data collected to 1.6 A resolution were used to determine the protein structure by the molecular replacement method. The fold of the protein comprises two structural domains: an alpha + beta N-terminal domain (residues 4-190) and a mainly alpha-helical C-terminal domain (residues 191-257). The active site is located in the interface between the two domains and comprises residues Val79, Tyr117, Glu167 and Arg170.
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<StructureSection load='2b7u' size='340' side='right'caption='[[2b7u]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2b7u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Squilla_maritima Squilla maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B7U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B7U FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b7u OCA], [https://pdbe.org/2b7u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b7u RCSB], [https://www.ebi.ac.uk/pdbsum/2b7u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b7u ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RIP_DRIMA RIP_DRIMA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b7/2b7u_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b7u ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2B7U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Charybdis_maritima Charybdis maritima] with <scene name='pdbligand=MES:'>MES</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B7U OCA].
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*[[Ribosome inactivating protein 3D structures|Ribosome inactivating protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Isolation, characterization, sequencing and crystal structure of charybdin, a type 1 ribosome-inactivating protein from Charybdis maritima agg., Touloupakis E, Gessmann R, Kavelaki K, Christofakis E, Petratos K, Ghanotakis DF, FEBS J. 2006 Jun;273(12):2684-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16817896 16817896]
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[[Category: Large Structures]]
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[[Category: Charybdis maritima]]
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[[Category: Squilla maritima]]
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[[Category: Protein complex]]
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[[Category: Gessmann R]]
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[[Category: rRNA N-glycosylase]]
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[[Category: Petratos K]]
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[[Category: Gessmann, R.]]
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[[Category: Petratos, K.]]
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[[Category: MES]]
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[[Category: ribosome inactivating protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:35:13 2008''
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Current revision

Ribosome inactivating protein type 1 from Charybdis maritima AGG

PDB ID 2b7u

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