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2b7y

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[[Image:2b7y.gif|left|200px]]<br /><applet load="2b7y" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2b7y, resolution 3.00&Aring;" />
 
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'''Fava Bean Lectin-Glucose Complex'''<br />
 
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==Overview==
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==Fava Bean Lectin-Glucose Complex==
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The three-dimensional structure of favin, the glucose- and mannose-binding lectin of Vicia faba (vetch, broad bean), has been determined at a resolution of 2.8 angstroms by molecular replacement. The crystals contain specifically bound glucose and provide the first high-resolution view of specific saccharide binding in a leguminous lectin. The structure is similar to those of concanavalin A (Con A) and green pea lectin; differences from Con A show that minimal changes are needed to accommodate the cyclic permutation in amino acid sequence between the two molecules. The molecule is an ellipsoidal dimer dominated by extensive beta structures. Each protomer contains binding sites for two divalent metal ions (Mn2+ and Ca2+) and a specific saccharide. Glucose is bound by favin in a cleft in the molecular surface and has noncovalent contacts primarily with two peptide loops, one of which contains several metal ion ligands. The specific carbohydrate-binding site is similar to that of Con A in location and general peptide folding, despite several differences in specific amino acid residues.
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<StructureSection load='2b7y' size='340' side='right'caption='[[2b7y]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2b7y]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Vicia_faba Vicia faba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B7Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B7Y FirstGlance]. <br>
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2B7Y is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Vicia_faba Vicia faba] with <scene name='pdbligand=GLC:'>GLC</scene>, <scene name='pdbligand=NDG:'>NDG</scene>, <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B7Y OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b7y OCA], [https://pdbe.org/2b7y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b7y RCSB], [https://www.ebi.ac.uk/pdbsum/2b7y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b7y ProSAT]</span></td></tr>
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Three-dimensional structure of favin: saccharide binding-cyclic permutation in leguminous lectins., Reeke GN Jr, Becker JW, Science. 1986 Nov 28;234(4780):1108-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=3775378 3775378]
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</table>
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[[Category: Protein complex]]
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== Function ==
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[https://www.uniprot.org/uniprot/LEC_VICFA LEC_VICFA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b7/2b7y_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b7y ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Vicia faba]]
[[Category: Vicia faba]]
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[[Category: Becker, J W.]]
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[[Category: Becker JW]]
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[[Category: Jr., G N.Reeke.]]
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[[Category: Reeke Jr GN]]
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[[Category: CA]]
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[[Category: GLC]]
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[[Category: MN]]
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[[Category: NDG]]
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[[Category: carbohydrate binding protein]]
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[[Category: d-glucose]]
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[[Category: glycoprotein]]
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[[Category: lectin-glucose complex]]
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[[Category: plant lectin]]
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[[Category: protein-carbohydrate complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:35:12 2008''
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Current revision

Fava Bean Lectin-Glucose Complex

PDB ID 2b7y

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