2w1p
From Proteopedia
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| - | {{STRUCTURE_2w1p| PDB=2w1p | SCENE= }} | ||
| - | ===1.4 Angstrom crystal structure of P.pastoris aquaporin, Aqy1, in a closed conformation at pH 8.0=== | ||
| - | {{ABSTRACT_PUBMED_19529756}} | ||
| - | == | + | ==1.4 Angstrom crystal structure of P.pastoris aquaporin, Aqy1, in a closed conformation at pH 8.0== |
| - | [[2w1p]] is a 1 chain structure with sequence from [ | + | <StructureSection load='2w1p' size='340' side='right'caption='[[2w1p]], [[Resolution|resolution]] 1.40Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2w1p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Komagataella_pastoris Komagataella pastoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W1P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W1P FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w1p OCA], [https://pdbe.org/2w1p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w1p RCSB], [https://www.ebi.ac.uk/pdbsum/2w1p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w1p ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/F2QVG4_KOMPC F2QVG4_KOMPC] | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w1/2w1p_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w1p ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Aquaporins are transmembrane proteins that facilitate the flow of water through cellular membranes. An unusual characteristic of yeast aquaporins is that they frequently contain an extended N terminus of unknown function. Here we present the X-ray structure of the yeast aquaporin Aqy1 from Pichia pastoris at 1.15 A resolution. Our crystal structure reveals that the water channel is closed by the N terminus, which arranges as a tightly wound helical bundle, with Tyr31 forming H-bond interactions to a water molecule within the pore and thereby occluding the channel entrance. Nevertheless, functional assays show that Aqy1 has appreciable water transport activity that aids survival during rapid freezing of P. pastoris. These findings establish that Aqy1 is a gated water channel. Mutational studies in combination with molecular dynamics simulations imply that gating may be regulated by a combination of phosphorylation and mechanosensitivity. | ||
| - | + | Crystal structure of a yeast aquaporin at 1.15 angstrom reveals a novel gating mechanism.,Fischer G, Kosinska-Eriksson U, Aponte-Santamaria C, Palmgren M, Geijer C, Hedfalk K, Hohmann S, de Groot BL, Neutze R, Lindkvist-Petersson K PLoS Biol. 2009 Jun;7(6):e1000130. Epub 2009 Jun 16. PMID:19529756<ref>PMID:19529756</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | < | + | </div> |
| + | <div class="pdbe-citations 2w1p" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Aquaporin 3D structures|Aquaporin 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Komagataella pastoris]] | [[Category: Komagataella pastoris]] | ||
| - | [[Category: Aponte-Santamaria | + | [[Category: Large Structures]] |
| - | [[Category: Fischer | + | [[Category: Aponte-Santamaria C]] |
| - | [[Category: Geijer | + | [[Category: Fischer G]] |
| - | + | [[Category: Geijer C]] | |
| - | [[Category: Hedfalk | + | [[Category: Hedfalk K]] |
| - | [[Category: Hohmann | + | [[Category: Hohmann S]] |
| - | [[Category: Kosinska-Eriksson | + | [[Category: Kosinska-Eriksson U]] |
| - | [[Category: Lindkvist-Petersson | + | [[Category: Lindkvist-Petersson K]] |
| - | [[Category: Neutze | + | [[Category: Neutze R]] |
| - | [[Category: Palmgren | + | [[Category: Palmgren M]] |
| - | [[Category: | + | [[Category: De Groot BL]] |
| - | + | ||
| - | + | ||
Current revision
1.4 Angstrom crystal structure of P.pastoris aquaporin, Aqy1, in a closed conformation at pH 8.0
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