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3ews

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{{STRUCTURE_3ews| PDB=3ews | SCENE= }}
 
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===Human DEAD-box RNA-helicase DDX19 in complex with ADP===
 
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{{ABSTRACT_PUBMED_19244245}}
 
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==About this Structure==
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==Human DEAD-box RNA-helicase DDX19 in complex with ADP==
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[[3ews]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EWS OCA].
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<StructureSection load='3ews' size='340' side='right'caption='[[3ews]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3ews]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EWS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ews FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ews OCA], [https://pdbe.org/3ews PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ews RCSB], [https://www.ebi.ac.uk/pdbsum/3ews PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ews ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DD19B_HUMAN DD19B_HUMAN] ATP-dependent RNA helicase involved in mRNA export from the nucleus. Rather than unwinding RNA duplexes, DDX19B functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/3ews_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ews ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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DEXD/H-box RNA helicases couple ATP hydrolysis to RNA remodeling by an unknown mechanism. We used x-ray crystallography and biochemical analysis of the human DEXD/H-box protein DDX19 to investigate its regulatory mechanism. The crystal structures of DDX19, in its RNA-bound prehydrolysis and free posthydrolysis state, reveal an alpha-helix that inserts between the conserved domains of the free protein to negatively regulate ATPase activity. This finding was corroborated by biochemical data that confirm an autoregulatory function of the N-terminal region of the protein. This is the first study describing crystal structures of a DEXD/H-box protein in its open and closed cleft conformations.
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==See Also==
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The DEXD/H-box RNA helicase DDX19 is regulated by an {alpha}-helical switch.,Collins R, Karlberg T, Lehtio L, Schutz P, van den Berg S, Dahlgren LG, Hammarstrom M, Weigelt J, Schuler H J Biol Chem. 2009 Apr 17;284(16):10296-300. Epub 2009 Feb 25. PMID:19244245<ref>PMID:19244245</ref>
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*[[DEAD-box RNA-helicase DDX19 in complex with ADP|DEAD-box RNA-helicase DDX19 in complex with ADP]]
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*[[Dead-box RNA helicase DDX19%2C in complex with an ATP-analogue and RNA|Dead-box RNA helicase DDX19%2C in complex with an ATP-analogue and RNA]]
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*[[Helicase|Helicase]]
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:019244245</ref><references group="xtra"/>
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</div>
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<div class="pdbe-citations 3ews" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Helicase 3D structures|Helicase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Andersson,j.]]
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[[Category: Large Structures]]
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[[Category: Arrowsmith,c h.]]
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[[Category: Andersson j]]
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[[Category: Berglund,h.]]
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[[Category: Arrowsmith ch]]
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[[Category: Bountra, c.]]
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[[Category: Berglund h]]
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[[Category: Collins,r.]]
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[[Category: Bountra c]]
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[[Category: Dahlgren,l g.]]
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[[Category: Collins r]]
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[[Category: Edwards,a m.]]
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[[Category: Dahlgren lg]]
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[[Category: Flodin,s.]]
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[[Category: Edwards am]]
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[[Category: Flores,a.]]
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[[Category: Flodin s]]
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[[Category: Graslund,s.]]
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[[Category: Flores a]]
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[[Category: Hammarstrom,m.]]
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[[Category: Graslund s]]
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[[Category: Johansson,a.]]
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[[Category: Hammarstrom m]]
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[[Category: Johansson,i.]]
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[[Category: Johansson a]]
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[[Category: Karlberg,t.]]
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[[Category: Johansson i]]
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[[Category: Kotenyova,t.]]
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[[Category: Karlberg t]]
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[[Category: Lehtio,l.]]
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[[Category: Kotenyova t]]
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[[Category: Moche,m.]]
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[[Category: Lehtio l]]
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[[Category: Nilsson,m e.]]
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[[Category: Moche m]]
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[[Category: Nordlund,p.]]
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[[Category: Nilsson me]]
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[[Category: Nyman,t.]]
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[[Category: Nordlund p]]
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[[Category: Olesen,k.]]
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[[Category: Nyman t]]
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[[Category: Persson,c.]]
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[[Category: Olesen k]]
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[[Category: SGC, Structural Genomics Consortium.]]
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[[Category: Persson c]]
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[[Category: Sagemark,j.]]
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[[Category: Sagemark j]]
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[[Category: Schueler,h.]]
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[[Category: Schueler h]]
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[[Category: Thorsell,a g.]]
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[[Category: Thorsell ag]]
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[[Category: Tresaugues,l.]]
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[[Category: Tresaugues l]]
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[[Category: Weigelt, j.]]
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[[Category: Van den berg s]]
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[[Category: Welin,m.]]
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[[Category: Weigelt j]]
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[[Category: Wikstrom,m.]]
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[[Category: Welin m]]
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[[Category: Wisniewska,m.]]
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[[Category: Wikstrom m]]
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[[Category: Berg, s Van den.]]
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[[Category: Wisniewska m]]
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[[Category: Adp]]
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[[Category: Atp-binding]]
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[[Category: Dead]]
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[[Category: Helicase]]
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[[Category: Hydrolase]]
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[[Category: Membrane]]
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[[Category: Mrna]]
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[[Category: Mrna transport]]
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[[Category: Nuclear pore complex]]
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[[Category: Nucleotide-binding]]
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[[Category: Nucleus]]
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[[Category: Protein transport]]
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[[Category: Rna helicase]]
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[[Category: Rna-binding]]
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[[Category: Rrna]]
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[[Category: Sgc]]
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[[Category: Structural genomic]]
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[[Category: Structural genomics consortium]]
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[[Category: Translocation]]
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[[Category: Transport]]
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Current revision

Human DEAD-box RNA-helicase DDX19 in complex with ADP

PDB ID 3ews

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