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3ere
From Proteopedia
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| - | {{STRUCTURE_3ere| PDB=3ere | SCENE= }} | ||
| - | ===Crystal structure of the arginine repressor protein from Mycobacterium tuberculosis in complex with the DNA operator=== | ||
| - | {{ABSTRACT_PUBMED_18952097}} | ||
| - | == | + | ==Crystal structure of the arginine repressor protein from Mycobacterium tuberculosis in complex with the DNA operator== |
| - | [[3ere]] is a 3 chain structure with sequence from [ | + | <StructureSection load='3ere' size='340' side='right'caption='[[3ere]], [[Resolution|resolution]] 2.50Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3ere]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ERE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ERE FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ere FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ere OCA], [https://pdbe.org/3ere PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ere RCSB], [https://www.ebi.ac.uk/pdbsum/3ere PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ere ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/ARGR_MYCTU ARGR_MYCTU] Regulates arginine biosynthesis genes (By similarity). | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/er/3ere_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ere ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The arginine repressor (ArgR) from Mycobacterium tuberculosis (Mtb) is a gene product encoded by the open reading frame Rv1657. It regulates the L-arginine concentration in cells by interacting with ARG boxes in the promoter regions of the arginine biosynthesis and catabolism operons. Here we present a 2.5-A structure of MtbArgR in complex with a 16-bp DNA operator in the absence of arginine. A biological trimer of the protein-DNA complex is formed via the crystallographic 3-fold symmetry axis. The N-terminal domain of MtbArgR has a winged helix-turn-helix motif that binds to the major groove of the DNA. This structure shows that, in the absence of arginine, the ArgR trimer can bind three ARG box half-sites. It also reveals the structure of the whole MtbArgR molecule itself containing both N-terminal and C-terminal domains. | ||
| + | |||
| + | Crystal structure of the arginine repressor protein in complex with the DNA operator from Mycobacterium tuberculosis.,Cherney LT, Cherney MM, Garen CR, Lu GJ, James MN J Mol Biol. 2008 Dec 31;384(5):1330-40. Epub 2008 Oct 15. PMID:18952097<ref>PMID:18952097</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3ere" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
| + | *[[Arginine repressor 3D structures|Arginine repressor 3D structures]] | ||
*[[Tomato aspermy virus protein 2b Suppression of RNA Silencing|Tomato aspermy virus protein 2b Suppression of RNA Silencing]] | *[[Tomato aspermy virus protein 2b Suppression of RNA Silencing|Tomato aspermy virus protein 2b Suppression of RNA Silencing]] | ||
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | < | + | </StructureSection> |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | + | [[Category: Mycobacterium tuberculosis H37Rv]] | |
| - | + | [[Category: Cherney LT]] | |
| - | + | [[Category: Cherney MM]] | |
| - | + | [[Category: Garen CR]] | |
| - | + | [[Category: James MN]] | |
| - | + | [[Category: Lu GJ]] | |
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| - | [[Category: Mycobacterium tuberculosis]] | + | |
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Current revision
Crystal structure of the arginine repressor protein from Mycobacterium tuberculosis in complex with the DNA operator
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