3hl2

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{{STRUCTURE_3hl2| PDB=3hl2 | SCENE= }}
 
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===The crystal structure of the human SepSecS-tRNASec complex===
 
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{{ABSTRACT_PUBMED_19608919}}
 
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==About this Structure==
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==The crystal structure of the human SepSecS-tRNASec complex==
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[[3hl2]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HL2 OCA].
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<StructureSection load='3hl2' size='340' side='right'caption='[[3hl2]], [[Resolution|resolution]] 2.81&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3hl2]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HL2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HL2 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.81&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLR:(5-HYDROXY-4,6-DIMETHYLPYRIDIN-3-YL)METHYL+DIHYDROGEN+PHOSPHATE'>PLR</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TS6:MONOTHIOPHOSPHATE'>TS6</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hl2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hl2 OCA], [https://pdbe.org/3hl2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hl2 RCSB], [https://www.ebi.ac.uk/pdbsum/3hl2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hl2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SPCS_HUMAN SPCS_HUMAN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hl/3hl2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hl2 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Selenocysteine is the only genetically encoded amino acid in humans whose biosynthesis occurs on its cognate transfer RNA (tRNA). O-Phosphoseryl-tRNA:selenocysteinyl-tRNA synthase (SepSecS) catalyzes the final step of selenocysteine formation by a poorly understood tRNA-dependent mechanism. The crystal structure of human tRNA(Sec) in complex with SepSecS, phosphoserine, and thiophosphate, together with in vivo and in vitro enzyme assays, supports a pyridoxal phosphate-dependent mechanism of Sec-tRNA(Sec) formation. Two tRNA(Sec) molecules, with a fold distinct from other canonical tRNAs, bind to each SepSecS tetramer through their 13-base pair acceptor-TPsiC arm (where Psi indicates pseudouridine). The tRNA binding is likely to induce a conformational change in the enzyme's active site that allows a phosphoserine covalently attached to tRNA(Sec), but not free phosphoserine, to be oriented properly for the reaction to occur.
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The human SepSecS-tRNASec complex reveals the mechanism of selenocysteine formation.,Palioura S, Sherrer RL, Steitz TA, Soll D, Simonovic M Science. 2009 Jul 17;325(5938):321-5. PMID:19608919<ref>PMID:19608919</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3hl2" style="background-color:#fffaf0;"></div>
==See Also==
==See Also==
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*[[Selenocysteine|Selenocysteine]]
 
*[[Selenocysteine synthase|Selenocysteine synthase]]
*[[Selenocysteine synthase|Selenocysteine synthase]]
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*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
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==Reference==
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== References ==
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<ref group="xtra">PMID:019608919</ref><references group="xtra"/>
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Palioura, S.]]
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[[Category: Large Structures]]
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[[Category: Simonovic, M]]
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[[Category: Palioura S]]
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[[Category: Soll, D.]]
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[[Category: Simonovic M]]
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[[Category: Steitz, T A.]]
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[[Category: Soll D]]
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[[Category: Protein biosynthesis]]
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[[Category: Steitz TA]]
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[[Category: Protein-rna complex]]
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[[Category: Pyridoxal phosphate]]
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[[Category: Selenium]]
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[[Category: Selenocysteine]]
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[[Category: Sepsec]]
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[[Category: Transferase]]
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[[Category: Trnasec]]
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Current revision

The crystal structure of the human SepSecS-tRNASec complex

PDB ID 3hl2

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Proteopedia Page Contributors and Editors (what is this?)

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