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3i5w

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{{STRUCTURE_3i5w| PDB=3i5w | SCENE= }}
 
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===Crystal structure of human alpha-defensin 5 (mutant R13H)===
 
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{{ABSTRACT_PUBMED_19589339}}
 
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==About this Structure==
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==Crystal structure of human alpha-defensin 5 (mutant R13H)==
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[[3i5w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I5W OCA].
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<StructureSection load='3i5w' size='340' side='right'caption='[[3i5w]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3i5w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I5W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I5W FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.63&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i5w OCA], [https://pdbe.org/3i5w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i5w RCSB], [https://www.ebi.ac.uk/pdbsum/3i5w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i5w ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DEF5_HUMAN DEF5_HUMAN] Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing.<ref>PMID:12021776</ref> <ref>PMID:15616305</ref> <ref>PMID:17088326</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Defensins constitute a major family of natural antimicrobial peptides that protect the host against microbial invasion. Here, we report on the antibacterial properties and cellular interaction of Human Defensin 5 as a function of its positive charge and hydrophobicity. We find that selective replacement of arginine residues in HD-5 by alanine or charge-neutral lysine residues reduces antibacterial killing as well as host cell interaction. We identify arginines at positions 9 and 28 in the HD-5 sequence as particularly important for its function. Replacement of arginine at position 13 to Histidine, as observed in a Crohn's disease patient, reduced bacterial killing strain-selectively. Finally, we find that HD-5 interacts with host cells via receptor-mediated mechanisms.
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==See Also==
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Selective arginines are important for the antibacterial activity and host cell interaction of human alpha-defensin 5.,de Leeuw E, Rajabi M, Zou G, Pazgier M, Lu W FEBS Lett. 2009 Aug 6;583(15):2507-12. Epub 2009 Jul 7. PMID:19589339<ref>PMID:19589339</ref>
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*[[Defensin|Defensin]]
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:019589339</ref><references group="xtra"/>
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</div>
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[[Category: Lu, W.]]
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<div class="pdbe-citations 3i5w" style="background-color:#fffaf0;"></div>
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[[Category: Pazgier, M.]]
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[[Category: Antibiotic]]
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==See Also==
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[[Category: Antimicrobial]]
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*[[Defensin 3D structures|Defensin 3D structures]]
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[[Category: Antimicrobial peptide]]
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== References ==
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[[Category: Antimicrobial protein]]
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<references/>
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[[Category: Defensin]]
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__TOC__
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[[Category: Disulfide bond]]
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</StructureSection>
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[[Category: Fungicide]]
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[[Category: Homo sapiens]]
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[[Category: Hd5]]
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[[Category: Large Structures]]
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[[Category: Human alpha-defensin 5]]
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[[Category: Lu W]]
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[[Category: Secreted]]
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[[Category: Pazgier M]]

Current revision

Crystal structure of human alpha-defensin 5 (mutant R13H)

PDB ID 3i5w

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