2x50

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{{STRUCTURE_2x50| PDB=2x50 | SCENE= }}
 
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===CRYSTAL STRUCTURE OF TRYPANOTHIONE REDUCTASE FROM LEISHMANIA INFANTUM IN COMPLEX WITH NADPH AND SILVER===
 
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==About this Structure==
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==Crystal structure of Trypanothione reductase from Leishmania infantum in complex with NADPH and silver==
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[[2x50]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_infantum Leishmania infantum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X50 OCA].
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<StructureSection load='2x50' size='340' side='right'caption='[[2x50]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2x50]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_infantum Leishmania infantum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X50 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X50 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AG:SILVER+ION'>AG</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x50 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x50 OCA], [https://pdbe.org/2x50 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x50 RCSB], [https://www.ebi.ac.uk/pdbsum/2x50 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x50 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A4HSF7_LEIIN A4HSF7_LEIIN]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In Leishmania the glutathione/glutathione reductase eukaryotic redox sys-tem is replaced by the unique trypanothione/trypanothione reductase (TR) system. In vitro, silver is a more effective TR inhibitor than antimony, the first line drug against leishmaniasis in most endemic countries, and its mechanism of inhibition is similar to that of Sb(III). In particular, silver binds with high affinity to the catalytic triad Cys52, Cys57, and His461', thereby inhibiting TR. Here, Ag(0) activity was tested on the promastigote and amastigote stages of Leishmania infantum using a drug-delivery system consisting in Ag(0) nanoparticles encapsulated by ferritin molecules (PfFt-AgNPs). These were able to induce an antiproliferative effect on the parasites at metal concentrations lower than those used with antimony.
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Inhibitory Effect of Silver Nanoparticles on Trypanothione Reductase Activity and Leishmania infantum Proliferation.,Baiocco P, Ilari A, Ceci P, Orsini S, Gramiccia M, Di Muccio T, Colotti G ACS Med Chem Lett. 2010 Dec 29;2(3):230-3. doi: 10.1021/ml1002629. eCollection, 2011 Mar 10. PMID:24900299<ref>PMID:24900299</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2x50" style="background-color:#fffaf0;"></div>
==See Also==
==See Also==
*[[Trypanothione reductase|Trypanothione reductase]]
*[[Trypanothione reductase|Trypanothione reductase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Leishmania infantum]]
[[Category: Leishmania infantum]]
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[[Category: Trypanothione-disulfide reductase]]
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[[Category: Baiocco P]]
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[[Category: Baiocco, P.]]
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[[Category: Colotti G]]
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[[Category: Colotti, G.]]
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[[Category: Ilari A]]
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[[Category: Ilari, A.]]
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[[Category: Fad]]
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[[Category: Flavoprotein]]
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[[Category: Leishmaniasis]]
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[[Category: Oxidative metabolism]]
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[[Category: Oxidoreductase]]
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[[Category: Redox-active center]]
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Current revision

Crystal structure of Trypanothione reductase from Leishmania infantum in complex with NADPH and silver

PDB ID 2x50

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