2a8v

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{{STRUCTURE_2a8v| PDB=2a8v | SCENE= }}
 
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===RHO TRANSCRIPTION TERMINATION FACTOR/RNA COMPLEX===
 
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{{ABSTRACT_PUBMED_10230401}}
 
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==About this Structure==
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==RHO TRANSCRIPTION TERMINATION FACTOR/RNA COMPLEX==
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[[2a8v]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8V OCA].
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<StructureSection load='2a8v' size='340' side='right'caption='[[2a8v]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2a8v]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A8V FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8v OCA], [https://pdbe.org/2a8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a8v RCSB], [https://www.ebi.ac.uk/pdbsum/2a8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a8v ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RHO_ECOLI RHO_ECOLI] Facilitates transcription termination by a mechanism that involves rho binding to the nascent RNA, activation of rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template. RNA-dependent NTPAse which utilizes all four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_01884]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a8/2a8v_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a8v ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The E. coli Rho protein disengages newly transcribed RNA from its DNA template, helping terminate certain transcripts. We have determined the X-ray crystal structure of the RNA-binding domain of Rho complexed to an RNA ligand. Filters that screen both ligand size and chemical functionality line the primary nucleic acid-binding site, imparting sequence specificity to a generic single-stranded nucleic acid-binding fold and explaining the preference of Rho for cytosine-rich RNA. The crystal packing reveals two Rho domain protomers bound to a single RNA with a single base spacer, suggesting that the strong RNA-binding sites of Rho may arise from pairing of RNA-binding modules. Dimerization of symmetric subunits on an asymmetric ligand is developed as a model for allosteric control in the action of the intact Rho hexamer.
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==See Also==
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The structural basis for terminator recognition by the Rho transcription termination factor.,Bogden CE, Fass D, Bergman N, Nichols MD, Berger JM Mol Cell. 1999 Apr;3(4):487-93. PMID:10230401<ref>PMID:10230401</ref>
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*[[Helicase|Helicase]]
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*[[Transcription Termination Factor Rho|Transcription Termination Factor Rho]]
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:010230401</ref><ref group="xtra">PMID:014636052</ref><ref group="xtra">PMID:015048824</ref><references group="xtra"/>
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</div>
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<div class="pdbe-citations 2a8v" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Helicase 3D structures|Helicase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Berger, J M.]]
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[[Category: Escherichia coli K-12]]
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[[Category: Bergman, N.]]
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[[Category: Large Structures]]
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[[Category: Bogden, C E.]]
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[[Category: Berger JM]]
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[[Category: Fass, D.]]
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[[Category: Bergman N]]
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[[Category: Nichols, M D.]]
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[[Category: Bogden CE]]
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[[Category: Ob fold]]
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[[Category: Fass D]]
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[[Category: Protein-rna complex]]
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[[Category: Nichols MD]]
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[[Category: Rho]]
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[[Category: Single-stranded nucleic acid binding domain]]
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Current revision

RHO TRANSCRIPTION TERMINATION FACTOR/RNA COMPLEX

PDB ID 2a8v

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