This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4irm

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (15:29, 20 September 2023) (edit) (undo)
 
(4 intermediate revisions not shown.)
Line 1: Line 1:
-
{{STRUCTURE_4irm| PDB=4irm | SCENE= }}
 
-
===Crystal structure of mntc r116a mutant exhibits flexibility in the c-terminal domain===
 
-
==About this Structure==
+
==Crystal structure of mntc r116a mutant exhibits flexibility in the c-terminal domain==
-
[[4irm]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp._pcc_6803 Synechocystis sp. pcc 6803]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3v63 3v63]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IRM OCA].
+
<StructureSection load='4irm' size='340' side='right'caption='[[4irm]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
-
[[Category: Synechocystis sp. pcc 6803]]
+
== Structural highlights ==
-
[[Category: Adir, N.]]
+
<table><tr><td colspan='2'>[[4irm]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3v63 3v63]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IRM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IRM FirstGlance]. <br>
-
[[Category: Kanteev, M.]]
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
-
[[Category: Manganese]]
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-
[[Category: Solute binding protein of abs transporter]]
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4irm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4irm OCA], [https://pdbe.org/4irm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4irm RCSB], [https://www.ebi.ac.uk/pdbsum/4irm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4irm ProSAT]</span></td></tr>
-
[[Category: Transport protein]]
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q79EF9_SYNY3 Q79EF9_SYNY3]
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The cyanobacterium Synechocystis sp. PCC 6803 imports Mn2+ ions via MntCAB, an ABC transport system that is expressed at submicromolar Mn2+ concentrations. The structures of the wild type (WT) and a site-directed mutant of the MntC solute-binding protein have been determined at 2.7 and 3.5 A resolution, respectively. The WT structure is significantly improved over the previously determined structure (PDB entry 1xvl), showing improved Mn2+ binding site parameters, disulfide bonds in all three monomers and ions bound to the protein surface, revealing the role of Zn2+ ions in the crystallization liquor. The structure of MntC reveals that the active site is surrounded by neutral-to-positive electrostatic potential and is dominated by a network of polar interactions centred around Arg116. The mutation of this residue to alanine was shown to destabilize loops in the entrance to the metal-ion binding site and suggests a possible role in MntC function.
 +
 
 +
Arginine 116 stabilizes the entrance to the metal ion-binding site of the MntC protein.,Kanteev M, Adir N Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Mar 1;69(Pt 3):237-42., doi: 10.1107/S174430911300153X. Epub 2013 Feb 22. PMID:23519795<ref>PMID:23519795</ref>
 +
 
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 4irm" style="background-color:#fffaf0;"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Synechocystis sp. PCC 6803]]
 +
[[Category: Adir N]]
 +
[[Category: Kanteev M]]

Current revision

Crystal structure of mntc r116a mutant exhibits flexibility in the c-terminal domain

PDB ID 4irm

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4irm"
Personal tools