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4jhu
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==T2-depleted laccase from Coriolopsis caperata soaked with CuCl== | |
| + | <StructureSection load='4jhu' size='340' side='right'caption='[[4jhu]], [[Resolution|resolution]] 1.89Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4jhu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Coriolopsis_caperata Coriolopsis caperata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JHU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JHU FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jhu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jhu OCA], [https://pdbe.org/4jhu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jhu RCSB], [https://www.ebi.ac.uk/pdbsum/4jhu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jhu ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/M1GME7_9APHY M1GME7_9APHY] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Laccases are members of a large family of multicopper oxidases that catalyze the oxidation of a wide range of organic and inorganic substrates accompanied by the reduction of dioxygen to water. A new laccase was isolated from the basidiomycete Coriolopsis caperata strain 0677 and its amino-acid sequence was determined. According to its physicochemical properties and spectroscopic features, the laccase from C. caperata is a high redox-potential blue laccase. Attempts to crystallize the native enzyme were unsuccessful. The copper type 2-depleted (T2D) laccase was prepared and crystallized. The structure of T2D laccase from C. caperata was solved at 1.6 A resolution, and attempts to reconstruct the T2 copper centre were performed using Cu(+) and Cu(2+) ions. The structure of T2D+Cu(+) laccase was solved at 1.89 A resolution. It was shown that the T2D+Cu(+) laccase structure contained four copper ions in the active site. Reconstruction could not be achieved when the T2D laccase crystals were treated with CuSO4. | ||
| - | + | Elucidation of the crystal structure of Coriolopsis caperata laccase: restoration of the structure and activity of the native enzyme from the T2-depleted form by copper ions.,Glazunova OA, Polyakov KM, Fedorova TV, Dorovatovskii PV, Koroleva OV Acta Crystallogr D Biol Crystallogr. 2015 Apr;71(Pt 4):854-61. doi:, 10.1107/S1399004715001595. Epub 2015 Mar 26. PMID:25849396<ref>PMID:25849396</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4jhu" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Laccase 3D structures|Laccase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Coriolopsis caperata]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Fedorova TV]] | ||
| + | [[Category: Glazunova OA]] | ||
| + | [[Category: Koroleva OA]] | ||
| + | [[Category: Kurzeev SA]] | ||
| + | [[Category: Maloshenok LG]] | ||
| + | [[Category: Polyakov KM]] | ||
Current revision
T2-depleted laccase from Coriolopsis caperata soaked with CuCl
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