3ie5

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{{STRUCTURE_3ie5| PDB=3ie5 | SCENE= }}
 
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===Crystal structure of Hyp-1 protein from Hypericum perforatum (St John's wort) involved in hypericin biosynthesis===
 
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{{ABSTRACT_PUBMED_19853038}}
 
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==About this Structure==
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==Crystal structure of Hyp-1 protein from Hypericum perforatum (St John's wort) involved in hypericin biosynthesis==
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[[3ie5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hypericum_perforatum Hypericum perforatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IE5 OCA].
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<StructureSection load='3ie5' size='340' side='right'caption='[[3ie5]], [[Resolution|resolution]] 1.69&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3ie5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Hypericum_perforatum Hypericum perforatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IE5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IE5 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.688&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PE8:3,6,9,12,15,18,21-HEPTAOXATRICOSANE-1,23-DIOL'>PE8</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ie5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ie5 OCA], [https://pdbe.org/3ie5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ie5 RCSB], [https://www.ebi.ac.uk/pdbsum/3ie5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ie5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8H1L1_HYPPE Q8H1L1_HYPPE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ie/3ie5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ie5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Hypericin, a red-colored naphtodianthrone, is a natural product synthesized in the medicinal plant Hypericum perforatum, widely known as St. John's wort. Hypericin has been attracting a growing attention of the pharmaceutical industry because of its potential application in various therapies, including the treatment of depression. In vivo, hypericin is synthesized by dimerization of emodin in a complicated multistep reaction that is reportedly catalyzed by a small (17.8kDa) protein, Hyp-1. Based on relatively low sequence similarity ( approximately 50%), Hyp-1 has been tentatively classified as a plant PR-10 (pathogenesis-related class 10) protein. Members of the PR-10 family are ubiquitous plant proteins associated with stress control and tissue differentiation but with no clearly understood molecular mechanism. They have, however, a well-defined folding canon, consisting of an extended antiparallel beta-sheet wrapped around a C-terminal alpha-helix, enclosing in the protein interior a huge cavity, in which various hydrophobic ligands can be bound. Apart from Hyp-1, only two other PR-10 members have been found to possess enzymatic activity (S-norcoclaurine synthase and TcmN aromatase/cyclase). In this paper, we report a high-resolution crystal structure of Hyp-1, confirming that it indeed has a PR-10 fold. The protein binds multiple polyethylene glycol molecules, some of which occupy the hydrophobic cavity. The crystallographic model illustrates a high degree of conformational adaptability of both interacting partners for efficient binding. We have been unable, however, to dimerize emodin to hypericin using Hyp-1 as biocatalyst. This puzzling result does not have a clear explanation at this time.
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==Reference==
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Crystal structure of Hyp-1, a St. John's wort protein implicated in the biosynthesis of hypericin.,Michalska K, Fernandes H, Sikorski M, Jaskolski M J Struct Biol. 2010 Feb;169(2):161-71. Epub 2009 Oct 21. PMID:19853038<ref>PMID:19853038</ref>
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<ref group="xtra">PMID:019853038</ref><ref group="xtra">PMID:018453712</ref><ref group="xtra">PMID:019220853</ref><ref group="xtra">PMID:018406424</ref><ref group="xtra">PMID:016998071</ref><ref group="xtra">PMID:015608381</ref><ref group="xtra">PMID:012079359</ref><ref group="xtra">PMID:008946858</ref><references group="xtra"/>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ie5" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Hypericum perforatum]]
[[Category: Hypericum perforatum]]
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[[Category: Fernandes, H.]]
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[[Category: Large Structures]]
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[[Category: Jaskolski, M.]]
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[[Category: Fernandes H]]
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[[Category: Michalska, K.]]
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[[Category: Jaskolski M]]
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[[Category: Sikorski, M M.]]
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[[Category: Michalska K]]
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[[Category: Allergy]]
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[[Category: Sikorski MM]]
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[[Category: Biosynthetic protein]]
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[[Category: Cytokinin]]
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[[Category: Depression]]
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[[Category: Hypericin]]
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[[Category: Pathogenesis-related protein]]
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[[Category: Peg]]
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[[Category: Plant defense]]
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[[Category: Plant hormone]]
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[[Category: Plant protein]]
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[[Category: Polyethylene glycol]]
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[[Category: Pr-10 protein]]
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[[Category: St john's wort]]
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Current revision

Crystal structure of Hyp-1 protein from Hypericum perforatum (St John's wort) involved in hypericin biosynthesis

PDB ID 3ie5

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