3i2w

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{{STRUCTURE_3i2w| PDB=3i2w | SCENE= }}
 
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===Crystal structure of EFC/F-BAR domain of Drosophila Syndapin/PACSIN===
 
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{{ABSTRACT_PUBMED_19997509}}
 
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==About this Structure==
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==Crystal structure of EFC/F-BAR domain of Drosophila Syndapin/PACSIN==
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[[3i2w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I2W OCA].
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<StructureSection load='3i2w' size='340' side='right'caption='[[3i2w]], [[Resolution|resolution]] 2.67&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3i2w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I2W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I2W FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CG33094, Dmel_CG33094, Synd, syndapin ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i2w OCA], [https://pdbe.org/3i2w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i2w RCSB], [https://www.ebi.ac.uk/pdbsum/3i2w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i2w ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i2/3i2w_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i2w ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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PACSIN/Syndapin proteins are membrane-active scaffolds that participate in endocytosis. The structure of the Drosophila Syndapin N-terminal EFC domain reveals a crescent shaped antiparallel dimer with a high affinity for phosphoinositides and a unique membrane-inserting prong upon the concave surface. Combined structural, biochemical and reverse genetic approaches in zebrafish define an important role for Syndapin orthologue, Pacsin3, in the early formation of the notochord during embryonic development. In pacsin3-morphant embryos, midline convergence of notochord precursors is defective as axial mesodermal cells fail to polarize, migrate and differentiate properly. The pacsin3 morphant phenotype of a stunted body axis and contorted trunk is rescued by ectopic expression of Drosophila Syndapin, and depends critically on both the prong that protrudes from the surface of the bowed Syndapin EFC domain and the ability of the antiparallel dimer to bind tightly to phosphoinositides. Our data confirm linkage between directional migration, endocytosis and cell specification during embryonic morphogenesis and highlight a key role for Pacsin3 in this coupling in the notochord.
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==Reference==
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Structural requirements for PACSIN/Syndapin operation during zebrafish embryonic notochord development.,Edeling MA, Sanker S, Shima T, Umasankar PK, Honing S, Kim HY, Davidson LA, Watkins SC, Tsang M, Owen DJ, Traub LM PLoS One. 2009 Dec 3;4(12):e8150. PMID:19997509<ref>PMID:19997509</ref>
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<ref group="xtra">PMID:019997509</ref><references group="xtra"/>
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[[Category: Drosophila melanogaster]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Edeling, M A.]]
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</div>
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[[Category: Owen, D J.]]
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<div class="pdbe-citations 3i2w" style="background-color:#fffaf0;"></div>
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[[Category: Traub, L M.]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Drome]]
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[[Category: Large Structures]]
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[[Category: Edeling, M A]]
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[[Category: Owen, D J]]
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[[Category: Traub, L M]]
[[Category: Efc]]
[[Category: Efc]]
[[Category: Endocytosis]]
[[Category: Endocytosis]]
[[Category: Fbar]]
[[Category: Fbar]]
[[Category: Sh3 domain]]
[[Category: Sh3 domain]]

Current revision

Crystal structure of EFC/F-BAR domain of Drosophila Syndapin/PACSIN

PDB ID 3i2w

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