2m5u
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 2m5u is ON HOLD Authors: Skilandat, M., Rowinska-Zyrek, M., Sigel, R.K.O. Description: NMR structure of the P4 hairpin of the CPEB3 ribozyme) |
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| - | '''Unreleased structure''' | ||
| - | + | ==NMR structure of the P4 hairpin of the CPEB3 ribozyme== | |
| + | <StructureSection load='2m5u' size='340' side='right'caption='[[2m5u]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2m5u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M5U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M5U FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m5u OCA], [https://pdbe.org/2m5u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m5u RCSB], [https://www.ebi.ac.uk/pdbsum/2m5u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m5u ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Three ribozymes are known to occur in humans, the CPEB3 ribozyme, the CoTC ribozyme, and the hammerhead ribozyme. Here, we present the NMR solution structure of a well-conserved motif within the CPEB3 ribozyme, the P4 domain. In addition, we discuss the binding sites and impact of Mg2+ and [Co(NH3)6]3+, a spectroscopic probe for [Mg(H2O)6]2+, on the structure. The well-defined P4 region is a hairpin closed with a UGGU tetraloop that shows a distinct electrostatic surface potential and a characteristic, strongly curved backbone trajectory. The P4 hairpin contains two specific Mg2+ binding sites: one outer-sphere binding site close to the proposed CPEB3 ribozyme active site with potential relevance for maintaining a compact fold of the ribozyme core, and one inner-sphere binding site, probably stabilizing the tetraloop structure. The structure of the tetraloop resembles an RNase III recognition structure, as previously described for an AGUU tetraloop. The detailed knowledge of the P4 domain and its metal ion binding preferences thus brings us closer to understanding the importance of Mg2+ binding for the CPEB3 ribozyme's fold and function in the cell. | ||
| - | + | Solution structure and metal ion binding sites of the human CPEB3 ribozyme's P4 domain.,Skilandat M, Rowinska-Zyrek M, Sigel RK J Biol Inorg Chem. 2014 Mar 21. PMID:24652468<ref>PMID:24652468</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 2m5u" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Rowinska-Zyrek M]] | ||
| + | [[Category: Sigel RKO]] | ||
| + | [[Category: Skilandat M]] | ||
Current revision
NMR structure of the P4 hairpin of the CPEB3 ribozyme
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