4jna
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4jna is ON HOLD Authors: Li, J., Wang, C., Zhang, Z.M., Zhou, J.H., Cheng, E. Description: Crystal structure of the DepH complex with dimethyl-FK22...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the DepH complex with dimethyl-FK228== | |
| + | <StructureSection load='4jna' size='340' side='right'caption='[[4jna]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4jna]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chromobacterium_violaceum Chromobacterium violaceum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JNA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JNA FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=060:S-METHYL-D-CYSTEINE'>060</scene>, <scene name='pdbligand=1KC:(3S)-7-METHYLSULFANYL-3-OXIDANYL-HEPT-4-ENOIC+ACID'>1KC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DBU:(2Z)-2-AMINOBUT-2-ENOIC+ACID'>DBU</scene>, <scene name='pdbligand=DVA:D-VALINE'>DVA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jna OCA], [https://pdbe.org/4jna PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jna RCSB], [https://www.ebi.ac.uk/pdbsum/4jna PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jna ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A4ZPY8_CHRVL A4ZPY8_CHRVL] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The disulfide bond is unusual in natural products and critical for thermal stability, cell permeability and bioactivity. DepH from Chromobacterium violaceum No. 968 is an FAD-dependent enzyme responsible for catalyzing the disulfide bond formation of FK228, an anticancer prodrug approved for the treatment of cutaneous T-cell lymphoma. Here we report the crystal structures of DepH and DepH complexed with a substrate analogue S,S'-dimethyl FK228 at 1.82 A and 2.00 A, respectively. Structural and biochemical analyses revealed that DepH, in contrast to the well characterized low molecular weight thioredoxin reductases (LMW TrxRs), is an NADP(+)-independent dithiol oxidase. DepH not only lacks a conserved GGGDXAXE motif necessary for NADP(+) binding in the canonical LMW TrxRs, but also contains a 11-residue sequence which physically impedes the binding of NADP(+). These observations explain the difference between NADP(+)-independent small molecule dithiol oxidases and NADP(+)-dependent thioredoxin reductases and provide insights for understanding the catalytic mechanism of dithiol oxidases involved in natural product biosynthesis. | ||
| - | + | The structural basis of an NADP(+)-independent dithiol oxidase in FK228 biosynthesis.,Li J, Wang C, Zhang ZM, Cheng YQ, Zhou J Sci Rep. 2014 Feb 20;4:4145. doi: 10.1038/srep04145. PMID:24553401<ref>PMID:24553401</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4jna" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Chromobacterium violaceum]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Cheng E]] | ||
| + | [[Category: Li J]] | ||
| + | [[Category: Wang C]] | ||
| + | [[Category: Zhang ZM]] | ||
| + | [[Category: Zhou JH]] | ||
Current revision
Crystal structure of the DepH complex with dimethyl-FK228
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