1oar

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[[Image:1oar.gif|left|200px]]<br />
 
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<applet load="1oar" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1oar, resolution 2.23&Aring;" />
 
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'''FV IGE SPE-7 IN COMPLEX WITH ALIZARIN RED'''<br />
 
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==Overview==
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==Fv IgE SPE-7 in complex with Alizarin Red==
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A single antibody was shown to adopt different binding-site conformations, and thereby bind unrelated antigens. Analysis by both x-ray, crystallography and pre-steady-state kinetics revealed an equilibrium, between different preexisting isomers, one of which possessed a, promiscuous, low-affinity binding site for aromatic ligands, including the, immunizing hapten. A subsequent induced-fit isomerization led to, high-affinity complexes with a deep and narrow binding site. A protein, antigen identified by repertoire selection made use of an unrelated, antibody isomer with a wide, shallow binding site. Conformational, diversity, whereby one sequence adopts multiple structures and multiple, functions, can increase the effective size of the antibody repertoire but, may also lead to autoimmunity ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12610298 (full description)]]
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<StructureSection load='1oar' size='340' side='right'caption='[[1oar]], [[Resolution|resolution]] 2.23&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1oar]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OAR FirstGlance]. <br>
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1OAR is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus]] with CAC, NA, CL, DMS, EDO and AZN as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OAR OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.23&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZN:ALIZARIN+RED'>AZN</scene>, <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oar OCA], [https://pdbe.org/1oar PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oar RCSB], [https://www.ebi.ac.uk/pdbsum/1oar PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oar ProSAT]</span></td></tr>
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Antibody multispecificity mediated by conformational diversity., James LC, Roversi P, Tawfik DS, Science. 2003 Feb 28;299(5611):1362-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12610298 12610298]
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</table>
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[[Category: Rattus rattus]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/LV1B_MOUSE LV1B_MOUSE]
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[[Category: James, L.C.]]
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== Evolutionary Conservation ==
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[[Category: Roversi, P.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Tawfik, D.]]
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Check<jmol>
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[[Category: AZN]]
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<jmolCheckbox>
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[[Category: CAC]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oa/1oar_consurf.spt"</scriptWhenChecked>
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[[Category: CL]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: DMS]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: EDO]]
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</jmolCheckbox>
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[[Category: NA]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oar ConSurf].
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[[Category: allergy]]
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<div style="clear:both"></div>
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[[Category: antibody]]
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__TOC__
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[[Category: conformational diversity]]
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</StructureSection>
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[[Category: ige]]
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[[Category: Large Structures]]
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[[Category: multispecificity]]
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[[Category: Mus musculus]]
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[[Category: James LC]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:48:23 2007''
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[[Category: Roversi P]]
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[[Category: Tawfik D]]

Current revision

Fv IgE SPE-7 in complex with Alizarin Red

PDB ID 1oar

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