2c5r

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:24, 6 September 2023) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2c5r.gif|left|200px]]<br /><applet load="2c5r" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2c5r, resolution 2.90&Aring;" />
 
-
'''THE STRUCTURE OF PHAGE PHI29 REPLICATION ORGANIZER PROTEIN P16.7 IN COMPLEX WITH DOUBLE STRANDED DNA'''<br />
 
-
==Overview==
+
==The structure of phage phi29 replication organizer protein p16.7 in complex with double stranded DNA==
 +
<StructureSection load='2c5r' size='340' side='right'caption='[[2c5r]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2c5r]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_virus_phi29 Bacillus virus phi29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C5R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C5R FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c5r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c5r OCA], [https://pdbe.org/2c5r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c5r RCSB], [https://www.ebi.ac.uk/pdbsum/2c5r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c5r ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/GP167_BPPH2 GP167_BPPH2] Binds to the long stretches of ssDNA of the viral DNA replication intermediates created during the protein-primed mechanism of replication of the viral genome and attaches the viral DNA to the membrane of the infected cells (PubMed:11741949) (PubMed:11169113). Required for the redistribution of replicating viral DNA from the initial replication site to membrane-associated sites surrounding the nucleoid (PubMed:10921898). Required for the second pull step of DNA ejection (PubMed:17526715).<ref>PMID:10921898</ref> <ref>PMID:11169113</ref> <ref>PMID:11741949</ref> <ref>PMID:17526715</ref>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
Prokaryotic DNA replication is compartmentalized at the cellular membrane. Functional and biochemical studies showed that the Bacillus subtilis phage 29-encoded membrane protein p16.7 is directly involved in the organization of membrane-associated viral DNA replication. The structure of the functional domain of p16.7 in complex with DNA, presented here, reveals the multimerization mode of the protein and provides insights in the organization of the phage genome at the membrane of the infected cell.
Prokaryotic DNA replication is compartmentalized at the cellular membrane. Functional and biochemical studies showed that the Bacillus subtilis phage 29-encoded membrane protein p16.7 is directly involved in the organization of membrane-associated viral DNA replication. The structure of the functional domain of p16.7 in complex with DNA, presented here, reveals the multimerization mode of the protein and provides insights in the organization of the phage genome at the membrane of the infected cell.
-
==About this Structure==
+
Structural basis for membrane anchorage of viral phi29 DNA during replication.,Albert A, Munoz-Espin D, Jimenez M, Asensio JL, Hermoso JA, Salas M, Meijer WJ J Biol Chem. 2005 Dec 30;280(52):42486-8. Epub 2005 Nov 7. PMID:16275651<ref>PMID:16275651</ref>
-
2C5R is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Vibrio_phage_f237 Vibrio phage f237]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C5R OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Structural basis for membrane anchorage of viral phi29 DNA during replication., Albert A, Munoz-Espin D, Jimenez M, Asensio JL, Hermoso JA, Salas M, Meijer WJ, J Biol Chem. 2005 Dec 30;280(52):42486-8. Epub 2005 Nov 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16275651 16275651]
+
</div>
-
[[Category: Protein complex]]
+
<div class="pdbe-citations 2c5r" style="background-color:#fffaf0;"></div>
-
[[Category: Vibrio phage f237]]
+
== References ==
-
[[Category: Albert, A.]]
+
<references/>
-
[[Category: Asensio, J L.]]
+
__TOC__
-
[[Category: Hermoso, J A.]]
+
</StructureSection>
-
[[Category: Jimenez, M.]]
+
[[Category: Bacillus virus phi29]]
-
[[Category: Meijer, W J.J.]]
+
[[Category: Large Structures]]
-
[[Category: Munoz-Espin, D.]]
+
[[Category: Albert A]]
-
[[Category: Salas, M.]]
+
[[Category: Asensio JL]]
-
[[Category: complex (dna-binding protein/dna)]]
+
[[Category: Hermoso JA]]
-
[[Category: dna-binding protein]]
+
[[Category: Jimenez M]]
-
 
+
[[Category: Meijer WJJ]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:45:11 2008''
+
[[Category: Munoz-Espin D]]
 +
[[Category: Salas M]]

Current revision

The structure of phage phi29 replication organizer protein p16.7 in complex with double stranded DNA

PDB ID 2c5r

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2c5r"
Personal tools