3bh6

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{{STRUCTURE_3bh6| PDB=3bh6 | SCENE= }}
 
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===Crystal structure of the RP2-Arl3 complex bound to GppNHp===
 
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{{ABSTRACT_PUBMED_018376416}}
 
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==Disease==
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==Crystal structure of the RP2-Arl3 complex bound to GppNHp==
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[[http://www.uniprot.org/uniprot/XRP2_HUMAN XRP2_HUMAN]] Defects in RP2 are the cause of retinitis pigmentosa type 2 (RP2) [MIM:[http://omim.org/entry/312600 312600]]; also known as X-linked retinitis pigmentosa 2 (XLRP-2). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.<ref>PMID:11847227</ref><ref>PMID:16472755</ref><ref>PMID:10942419</ref><ref>PMID:9697692</ref><ref>PMID:10090907</ref><ref>PMID:10520237</ref><ref>PMID:10634633</ref><ref>PMID:10937588</ref><ref>PMID:11462235</ref><ref>PMID:11992260</ref><ref>PMID:14564670</ref><ref>PMID:12657579</ref><ref>PMID:22334370</ref>
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<StructureSection load='3bh6' size='340' side='right'caption='[[3bh6]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3bh6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BH6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bh6 OCA], [https://pdbe.org/3bh6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bh6 RCSB], [https://www.ebi.ac.uk/pdbsum/3bh6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bh6 ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/XRP2_HUMAN XRP2_HUMAN] Defects in RP2 are the cause of retinitis pigmentosa type 2 (RP2) [MIM:[https://omim.org/entry/312600 312600]; also known as X-linked retinitis pigmentosa 2 (XLRP-2). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.<ref>PMID:11847227</ref> <ref>PMID:16472755</ref> <ref>PMID:10942419</ref> <ref>PMID:9697692</ref> <ref>PMID:10090907</ref> <ref>PMID:10520237</ref> <ref>PMID:10634633</ref> <ref>PMID:10937588</ref> <ref>PMID:11462235</ref> <ref>PMID:11992260</ref> <ref>PMID:14564670</ref> <ref>PMID:12657579</ref> <ref>PMID:22334370</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/XRP2_HUMAN XRP2_HUMAN] Acts as a GTPase-activating protein (GAP) involved in trafficking between the Golgi and the ciliary membrane. Involved in localization of proteins, such as NPHP3, to the cilium membrane by inducing hydrolysis of GTP ARL3, leading to the release of UNC119 (or UNC119B). Acts as a GTPase-activating protein (GAP) for tubulin in concert with tubulin-specific chaperone C, but does not enhance tubulin heterodimerization. Acts as guanine nucleotide dissociation inhibitor towards ADP-ribosylation factor-like proteins.<ref>PMID:11847227</ref> <ref>PMID:20106869</ref> <ref>PMID:22085962</ref> <ref>PMID:18376416</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bh/3bh6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bh6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The retinitis pigmentosa 2 (RP2) gene is responsible for a particular variant of X chromosome-linked eye disease. Previously, RP2 was shown to bind the GTP form of the small G protein Arf-like 3 (Arl3), thus qualifying as an effector. Here we present the Arl3-GppNHp-RP2 complex structure, which shows features resembling complexes with GTPase-activating proteins (GAPs). Biochemical analysis showing a 90,000-fold stimulation of the GTPase reaction together with the structure of an Arl3-GDP-AlF4--RP2 transition state complex showed that RP2 is an efficient GAP for Arl3, with structural features similar to other GAPs. Furthermore, the effect of mutations in patients with retinitis pigmentosa correlated with their effect on catalysis, in particular the mutation of the arginine finger of RP2. The cognate G protein-GAP pair is conserved in yeast as Cin4-Cin2, and the ability of RP2 to act as a GAP can be correlated with its ability to complement a CIN2-deletion phenotype.
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==Function==
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The retinitis pigmentosa 2 gene product is a GTPase-activating protein for Arf-like 3.,Veltel S, Gasper R, Eisenacher E, Wittinghofer A Nat Struct Mol Biol. 2008 Apr;15(4):373-80. Epub 2008 Mar 23. PMID:18376416<ref>PMID:18376416</ref>
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[[http://www.uniprot.org/uniprot/ARL3_MOUSE ARL3_MOUSE]] Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium (By similarity). Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms.<ref>PMID:15979089</ref><ref>PMID:18376416</ref> [[http://www.uniprot.org/uniprot/XRP2_HUMAN XRP2_HUMAN]] Acts as a GTPase-activating protein (GAP) involved in trafficking between the Golgi and the ciliary membrane. Involved in localization of proteins, such as NPHP3, to the cilium membrane by inducing hydrolysis of GTP ARL3, leading to the release of UNC119 (or UNC119B). Acts as a GTPase-activating protein (GAP) for tubulin in concert with tubulin-specific chaperone C, but does not enhance tubulin heterodimerization. Acts as guanine nucleotide dissociation inhibitor towards ADP-ribosylation factor-like proteins.<ref>PMID:11847227</ref><ref>PMID:20106869</ref><ref>PMID:22085962</ref><ref>PMID:18376416</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3bh6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BH6 OCA].
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</div>
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<div class="pdbe-citations 3bh6" style="background-color:#fffaf0;"></div>
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==Reference==
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== References ==
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<references group="xtra"/><references/>
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Gasper, R.]]
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[[Category: Gasper R]]
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[[Category: Veltel, S.]]
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[[Category: Veltel S]]
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[[Category: Wittinghofer, A.]]
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[[Category: Wittinghofer A]]
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[[Category: Disease mutation]]
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[[Category: Gtp-binding]]
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[[Category: Gtpase activating protein and gtpase]]
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[[Category: Lipoprotein]]
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[[Category: Membrane]]
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[[Category: Metal binding protein]]
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[[Category: Myristate]]
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[[Category: Nucleotide-binding]]
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[[Category: Palmitate]]
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[[Category: Phosphoprotein]]
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[[Category: Protein-protein complex]]
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[[Category: Retinitis pigmentosa]]
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[[Category: Sensory transduction]]
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[[Category: Signaling protein]]
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[[Category: Vision]]
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Current revision

Crystal structure of the RP2-Arl3 complex bound to GppNHp

PDB ID 3bh6

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