2cmm

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[[Image:2cmm.jpg|left|200px]]<br /><applet load="2cmm" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2cmm, resolution 1.8&Aring;" />
 
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'''STRUCTURAL ANALYSIS OF THE MYOGLOBIN RECONSTITUTED WITH IRON PORPHINE'''<br />
 
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==Overview==
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==STRUCTURAL ANALYSIS OF THE MYOGLOBIN RECONSTITUTED WITH IRON PORPHINE==
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Sperm whale apomyoglobin was complexed with iron porphine to examine the influence of completely removed heme side chains on the entire molecular structure. Paramagnetic NMR peak from the proximal histidine of the deoxy protein ensured formation of the iron-histidine bond. Porphine pyrrole-proton NMR signals of the cyanmet and deoxy derivatives are unusually sharp single lines manifesting rapid heme rotation about the iron-histidine bond. X-ray crystallographic structure of the cyanmet derivative, determined with a final R factor of 0.21 for 11,808 independent reflections ranging from 7 to 1.8 A, was resolved at 1.8 A resolution. The result confirmed 1:1 coupling between apomyoglobin and iron porphine. The cyano ligand adopts a bent configuration with an Fe-C-N angle of 127 degrees and a Fe-CN distance of 1.89 A. The overall globin structure and side chain conformations are remarkably similar to those of native myoglobin despite intensive disruption of the original heme-globin interactions. The native apoprotein structure unexpectedly conserved even after iron porphine insertion demonstrates that the complex polypeptide fold of holomyoglobin is more inherent in the amino acid sequence than is generally believed.
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<StructureSection load='2cmm' size='340' side='right'caption='[[2cmm]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2cmm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CMM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CMM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene>, <scene name='pdbligand=POR:PORPHYRIN+FE(III)'>POR</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cmm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cmm OCA], [https://pdbe.org/2cmm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cmm RCSB], [https://www.ebi.ac.uk/pdbsum/2cmm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cmm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MYG_PHYMC MYG_PHYMC] Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cm/2cmm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cmm ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2CMM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon] with <scene name='pdbligand=CYN:'>CYN</scene> and <scene name='pdbligand=POR:'>POR</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CMM OCA].
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*[[Myoglobin 3D structures|Myoglobin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural analysis of the myoglobin reconstituted with iron porphine., Neya S, Funasaki N, Sato T, Igarashi N, Tanaka N, J Biol Chem. 1993 Apr 25;268(12):8935-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8473336 8473336]
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[[Category: Large Structures]]
[[Category: Physeter catodon]]
[[Category: Physeter catodon]]
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[[Category: Single protein]]
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[[Category: Funasaki N]]
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[[Category: Funasaki, N.]]
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[[Category: Igarashi N]]
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[[Category: Igarashi, N.]]
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[[Category: Iizuka T]]
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[[Category: Iizuka, T.]]
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[[Category: Moriyama H]]
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[[Category: Moriyama, H.]]
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[[Category: Neya S]]
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[[Category: Neya, S.]]
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[[Category: Sato T]]
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[[Category: Sato, T.]]
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[[Category: Shiro Y]]
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[[Category: Shiro, Y.]]
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[[Category: Tanaka N]]
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[[Category: Tanaka, N.]]
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[[Category: CYN]]
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[[Category: POR]]
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[[Category: oxygen transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:50:15 2008''
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Current revision

STRUCTURAL ANALYSIS OF THE MYOGLOBIN RECONSTITUTED WITH IRON PORPHINE

PDB ID 2cmm

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