2cny

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[[Image:2cny.gif|left|200px]]<br /><applet load="2cny" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2cny, resolution 1.90&Aring;" />
 
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'''SALMONELLA ENTERICA SAFA PILIN IN COMPLEX WITH A 19-RESIDUE SAFA NTE PEPTIDE (I15A MUTANT)'''<br />
 
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==Overview==
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==Salmonella enterica SafA pilin in complex with a 19-residue SafA Nte peptide (I15A mutant)==
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Gram-negative pathogens commonly use the chaperone-usher pathway to assemble adhesive multisubunit fibers on their surface. In the periplasm, subunits are stabilized by a chaperone that donates a beta strand to complement the subunits' truncated immunoglobulin-like fold. Pilus assembly proceeds through a "donor-strand exchange" (DSE) mechanism whereby this complementary beta strand is replaced by the N-terminal extension (Nte) of an incoming pilus subunit. Using X-ray crystallography and real-time electrospray ionization mass spectrometry (ESI-MS), we demonstrate that DSE requires the formation of a transient ternary complex between the chaperone-subunit complex and the Nte of the next subunit to be assembled. The process is crucially dependent on an initiation site (the P5 pocket) needed to recruit the incoming Nte. The data also suggest a capping reaction displacing DSE toward product formation. These results support a zip-in-zip-out mechanism for DSE and a catalytic role for the usher, the molecular platform at which pili are assembled.
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<StructureSection load='2cny' size='340' side='right'caption='[[2cny]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2cny]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CNY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CNY FirstGlance]. <br>
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2CNY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CNY OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cny FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cny OCA], [https://pdbe.org/2cny PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cny RCSB], [https://www.ebi.ac.uk/pdbsum/2cny PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cny ProSAT]</span></td></tr>
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==Reference==
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</table>
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Donor-strand exchange in chaperone-assisted pilus assembly proceeds through a concerted beta strand displacement mechanism., Remaut H, Rose RJ, Hannan TJ, Hultgren SJ, Radford SE, Ashcroft AE, Waksman G, Mol Cell. 2006 Jun 23;22(6):831-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16793551 16793551]
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== Function ==
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[[Category: Protein complex]]
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[https://www.uniprot.org/uniprot/Q8ZRK4_SALTY Q8ZRK4_SALTY]
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[[Category: Salmonella typhimurium]]
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__TOC__
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[[Category: Ashcroft, A E.]]
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</StructureSection>
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[[Category: Hannan, T J.]]
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[[Category: Large Structures]]
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[[Category: Hultgren, S J.]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Radford, S E.]]
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[[Category: Ashcroft AE]]
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[[Category: Remaut, H.]]
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[[Category: Hannan TJ]]
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[[Category: Rose, R J.]]
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[[Category: Hultgren SJ]]
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[[Category: Waksman, G.]]
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[[Category: Radford SE]]
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[[Category: fibril protein]]
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[[Category: Remaut H]]
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[[Category: fold complementation]]
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[[Category: Rose RJ]]
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[[Category: pilus subunit]]
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[[Category: Waksman G]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:50:40 2008''
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Current revision

Salmonella enterica SafA pilin in complex with a 19-residue SafA Nte peptide (I15A mutant)

PDB ID 2cny

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