2cs7

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[[Image:2cs7.gif|left|200px]]<br /><applet load="2cs7" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2cs7, resolution 1.20&Aring;" />
 
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'''1.2 A Crystal structure of the S. pneumoniae PhtA histidine triad domain a novel zinc binding fold'''<br />
 
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==Overview==
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==1.2 A Crystal structure of the S. pneumoniae PhtA histidine triad domain a novel zinc binding fold==
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The recently described pneumococcal histidine triad protein family has been shown to be highly conserved within the pneumococcus. As part of our structural genomics effort on proteins from Streptococcus pneumoniae, we have expressed, crystallised and solved the structure of PhtA-166-220 at 1.2 Angstroms using remote SAD with zinc. The structure of PhtA-166-220 shows no similarity to any protein structure. The overall fold contains 3beta-strands and a single short alpha-helix. The structure appears to contain a novel zinc binding motif. The remaining 4 histidine triad repeats from PhtA have been modelled based on the crystal structure of the PhtA histidine triad repeat 2. From this modelling work, we speculate that only three of the five histidine triad repeats contain the residues in the correct geometry to allow the binding of a zinc ion.
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<StructureSection load='2cs7' size='340' side='right'caption='[[2cs7]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2cs7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CS7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CS7 FirstGlance]. <br>
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2CS7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CS7 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cs7 OCA], [https://pdbe.org/2cs7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cs7 RCSB], [https://www.ebi.ac.uk/pdbsum/2cs7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cs7 ProSAT]</span></td></tr>
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1.2 Angstroms crystal structure of the S. pneumoniae PhtA histidine triad domain a novel zinc binding fold., Riboldi-Tunnicliffe A, Isaacs NW, Mitchell TJ, FEBS Lett. 2005 Oct 10;579(24):5353-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16194532 16194532]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/A0A0H2UQ89_STRPN A0A0H2UQ89_STRPN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cs/2cs7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cs7 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptococcus pneumoniae]]
[[Category: Streptococcus pneumoniae]]
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[[Category: Isaacs, N W.]]
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[[Category: Isaacs NW]]
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[[Category: Mitchell, T J.]]
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[[Category: Mitchell TJ]]
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[[Category: Riboldi-Tunnicliffe, A.]]
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[[Category: Riboldi-Tunnicliffe A]]
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[[Category: ZN]]
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[[Category: phta]]
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[[Category: pneumococcal histidine triad protein]]
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[[Category: s pneumoniae]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:51:53 2008''
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Current revision

1.2 A Crystal structure of the S. pneumoniae PhtA histidine triad domain a novel zinc binding fold

PDB ID 2cs7

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