2cxc

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[[Image:2cxc.gif|left|200px]]<br /><applet load="2cxc" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2cxc, resolution 2.00&Aring;" />
 
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'''Crystal structure of archaeal transcription termination factor NusA'''<br />
 
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==Overview==
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==Crystal structure of archaeal transcription termination factor NusA==
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The transcription factor NusA functions in transcriptional regulation involving termination in bacteria. A NusA homolog consisting of only the two KH domains is widely conserved in archaea, but its function remains unknown. We have found that Aeropyrum pernix NusA strongly binds to a certain CU-rich sequence near a termination signal. Our crystal structure of A. pernix NusA revealed that its spatial arrangement is quite similar to that of the KH domains of bacterial NusA. Thus, we consider archaeal NusA to have retained some functions of bacterial NusA, including the ssRNA-binding ability. Remarkable structural differences between archaeal and bacterial NusA exist at the interface with RNAP, in connection with the different NusA-binding sites around the termination signals. Transcriptional termination in archaea could differ from all of the known bacterial and eukaryal mechanisms, in terms of the combination of a bacterial factor and a eukaryal-type RNAP.
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<StructureSection load='2cxc' size='340' side='right'caption='[[2cxc]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2cxc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeropyrum_pernix_K1 Aeropyrum pernix K1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CXC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CXC FirstGlance]. <br>
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2CXC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aeropyrum_pernix Aeropyrum pernix]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CXC OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cxc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cxc OCA], [https://pdbe.org/2cxc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cxc RCSB], [https://www.ebi.ac.uk/pdbsum/2cxc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cxc ProSAT], [https://www.topsan.org/Proteins/RSGI/2cxc TOPSAN]</span></td></tr>
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==Reference==
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</table>
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Crystal structure and RNA-binding analysis of the archaeal transcription factor NusA., Shibata R, Bessho Y, Shinkai A, Nishimoto M, Fusatomi E, Terada T, Shirouzu M, Yokoyama S, Biochem Biophys Res Commun. 2007 Mar 30;355(1):122-8. Epub 2007 Jan 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17288993 17288993]
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== Function ==
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[[Category: Aeropyrum pernix]]
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[https://www.uniprot.org/uniprot/Q9YAU4_AERPE Q9YAU4_AERPE]
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Bessho, Y.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
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Check<jmol>
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[[Category: Shibata, R.]]
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<jmolCheckbox>
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[[Category: Shirouzu, M.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cx/2cxc_consurf.spt"</scriptWhenChecked>
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[[Category: Yokoyama, S.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: archaeal nusa]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: kh domain]]
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</jmolCheckbox>
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[[Category: national project on protein structural and functional analyses]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cxc ConSurf].
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[[Category: nppsfa]]
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<div style="clear:both"></div>
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[[Category: riken structural genomics/proteomics initiative]]
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__TOC__
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[[Category: rna binding protein]]
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</StructureSection>
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[[Category: rsgi]]
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[[Category: Aeropyrum pernix K1]]
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[[Category: structural genomics]]
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[[Category: Large Structures]]
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[[Category: transcription termination]]
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[[Category: Bessho Y]]
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[[Category: Shibata R]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:53:20 2008''
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[[Category: Shirouzu M]]
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[[Category: Yokoyama S]]

Current revision

Crystal structure of archaeal transcription termination factor NusA

PDB ID 2cxc

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